Min Kyu Kang1, Ok Joon Kim2, Young Joo Jeon3, Hyun Sook Kim2, Seung Hun Oh2, Jin Kwon Kim2, Eo Jin Kim4, Tae Sun Hwang5, Nam Keun Kim6. 1. Department of Biomedical Science, College of Life Science, CHA University, Republic of Korea. 2. Department of Neurology, School of Medicine, CHA University, Seongnam, Republic of Korea. 3. The Institute for Clinical Research, School of Medicine, CHA University, Seongnam, Republic of Korea. 4. Department of Medicine, College of Medicine, Chung-Ang University, Seoul, Republic of Korea. 5. Department of Anatomy, School of Medicine, CHA University, Seongnam, Republic of Korea. Electronic address: tshwang@cha.ac.kr. 6. Department of Biomedical Science, College of Life Science, CHA University, Republic of Korea; The Institute for Clinical Research, School of Medicine, CHA University, Seongnam, Republic of Korea. Electronic address: nkkim@cha.ac.kr.
Abstract
BACKGROUND: Endothelial nitric oxide synthase (eNOS) gene variants are known to play a role in atherosclerotic development. However, whether interplay between eNOS polymorphisms and metabolic syndrome (MetS) affects ischemic stroke (IS) risk has yet to be discovered. We investigated whether the combined effects of eNOS polymorphisms and MetS influence ischemic stroke risk in Koreans. METHODS: We genotyped the eNOS -922A>G, -786T>C, 4a4b, and 894G>T polymorphisms in 531 IS cases and 502 controls using polymerase chain reaction-amplified DNA. We then investigated whether the presence of MetS and the number of MetS risk factors worked with eNOS polymorphisms to influence IS risk. RESULTS: IS patients had a significantly higher prevalence of MetS than controls [adjusted odds ratio (AOR)=2.943, 95% confidence interval (CI), 2.256-3.840, P<0.0001], and MetS prevalence did not differ between stroke subtypes. The 894GT+TT genotypes were positively associated with IS (AOR=1.670, 95% CI, 1.208-2.308, P=0.002), and the -786TC+CC genotypes showed co-morbidity with MetS (AOR=1.448, 95% CI, 1.401-2.015, P=0.028). Among subjects with three or more MetS risk factors, the highest AOR value (28.490, 95% CI, 3.162-256.688) was observed for the eNOS -786TC+CC genotypes. CONCLUSIONS: The eNOS 894T allele and interplay between the eNOS -786C allele and MetS may predispose Koreans to IS.
BACKGROUND:Endothelial nitric oxide synthase (eNOS) gene variants are known to play a role in atherosclerotic development. However, whether interplay between eNOS polymorphisms and metabolic syndrome (MetS) affects ischemic stroke (IS) risk has yet to be discovered. We investigated whether the combined effects of eNOS polymorphisms and MetS influence ischemic stroke risk in Koreans. METHODS: We genotyped the eNOS -922A>G, -786T>C, 4a4b, and 894G>T polymorphisms in 531 IS cases and 502 controls using polymerase chain reaction-amplified DNA. We then investigated whether the presence of MetS and the number of MetS risk factors worked with eNOS polymorphisms to influence IS risk. RESULTS: IS patients had a significantly higher prevalence of MetS than controls [adjusted odds ratio (AOR)=2.943, 95% confidence interval (CI), 2.256-3.840, P<0.0001], and MetS prevalence did not differ between stroke subtypes. The 894GT+TT genotypes were positively associated with IS (AOR=1.670, 95% CI, 1.208-2.308, P=0.002), and the -786TC+CC genotypes showed co-morbidity with MetS (AOR=1.448, 95% CI, 1.401-2.015, P=0.028). Among subjects with three or more MetS risk factors, the highest AOR value (28.490, 95% CI, 3.162-256.688) was observed for the eNOS -786TC+CC genotypes. CONCLUSIONS: The eNOS 894T allele and interplay between the eNOS -786C allele and MetS may predispose Koreans to IS.
Authors: Eun Ju Ko; Eo Jin Kim; Hye Jung Cho; Jisu Oh; Han Sung Park; Chang Soo Ryu; Jung Oh Kim; Hak Hoon Jun; So Young Chong; Jong Woo Kim; Nam Keun Kim Journal: Genes Genomics Date: 2022-04-04 Impact factor: 2.164