AIM: Variations in the expression of cytokines during the progression of periodontitis remain ill-defined. We evaluated the expression of 19 cytokine genes related to T-cell phenotype/function during initiation, progression and resolution of periodontitis, and related these to the expression of soft and bone tissue destruction genes (TDGs). MATERIALS AND METHODS: A ligature-induced periodontitis model was used in rhesus monkeys (M. mulatta) (n = 18). Gingival tissues were taken at baseline pre-ligation, 2 weeks and 1 month (Initiation) and 3 months (progression) post ligation. Ligatures were removed and samples taken 2 months later (resolution). Total RNA was isolated and the Rhesus Gene 1.0 ST (Affymetrix) used for gene expression analysis. Significant expression changes were validated by qRT-PCR. RESULTS: Disease initiation/progression was characterized by overexpression of Th17/Treg cytokine genes (IL-1β, IL-6, TGFβ and IL-21) and down-regulation of Th1/Th2 cytokine genes (IL-18 and IL-25). Increased IL-2 and decreased IL-10 levels were seen during disease resolution. Several Th17/Treg cytokine genes positively correlated with TDGs, whereas most Th1/Th2 genes exhibited a negative correlation. CONCLUSION: Initiation, progression and resolution of periodontitis involve over- and underexpression of cytokine genes related to various T-helper subsets. In addition, variations in individual T-helper response subset/genes during disease progression correlated with protective/destructive outcomes.
AIM: Variations in the expression of cytokines during the progression of periodontitis remain ill-defined. We evaluated the expression of 19 cytokine genes related to T-cell phenotype/function during initiation, progression and resolution of periodontitis, and related these to the expression of soft and bone tissue destruction genes (TDGs). MATERIALS AND METHODS: A ligature-induced periodontitis model was used in rhesus monkeys (M. mulatta) (n = 18). Gingival tissues were taken at baseline pre-ligation, 2 weeks and 1 month (Initiation) and 3 months (progression) post ligation. Ligatures were removed and samples taken 2 months later (resolution). Total RNA was isolated and the Rhesus Gene 1.0 ST (Affymetrix) used for gene expression analysis. Significant expression changes were validated by qRT-PCR. RESULTS: Disease initiation/progression was characterized by overexpression of Th17/Treg cytokine genes (IL-1β, IL-6, TGFβ and IL-21) and down-regulation of Th1/Th2 cytokine genes (IL-18 and IL-25). Increased IL-2 and decreased IL-10 levels were seen during disease resolution. Several Th17/Treg cytokine genes positively correlated with TDGs, whereas most Th1/Th2 genes exhibited a negative correlation. CONCLUSION: Initiation, progression and resolution of periodontitis involve over- and underexpression of cytokine genes related to various T-helper subsets. In addition, variations in individual T-helper response subset/genes during disease progression correlated with protective/destructive outcomes.
Authors: Grishondra L Branch-Mays; Dolphus R Dawson; John C Gunsolley; Mark A Reynolds; Jeffrey L Ebersole; Karen F Novak; Julie A Mattison; Donald K Ingram; M John Novak Journal: J Periodontol Date: 2008-07 Impact factor: 6.993
Authors: Davide Agnello; Carla S R Lankford; Jay Bream; Akio Morinobu; Massimo Gadina; John J O'Shea; David M Frucht Journal: J Clin Immunol Date: 2003-05 Impact factor: 8.317
Authors: J P van Hamburg; P S Asmawidjaja; N Davelaar; A M C Mus; E M Colin; J M W Hazes; R J E M Dolhain; E Lubberts Journal: Arthritis Rheum Date: 2011-01
Authors: Alexander M Owyang; Colby Zaph; Emma H Wilson; Katherine J Guild; Terrill McClanahan; Hugh R P Miller; Daniel J Cua; Michael Goldschmidt; Christopher A Hunter; Robert A Kastelein; David Artis Journal: J Exp Med Date: 2006-04-10 Impact factor: 14.307
Authors: O A Gonzalez; M J Novak; S Kirakodu; A Stromberg; R Nagarajan; C B Huang; K C Chen; L Orraca; J Martinez-Gonzalez; J L Ebersole Journal: Immunol Invest Date: 2015-09-23 Impact factor: 3.657
Authors: Tomoki Maekawa; Ruel A Briones; Ranillo R G Resuello; Joel V Tuplano; Evlambia Hajishengallis; Tetsuhiro Kajikawa; Sophia Koutsogiannaki; Cristina A G Garcia; Daniel Ricklin; John D Lambris; George Hajishengallis Journal: J Clin Periodontol Date: 2016-03-03 Impact factor: 8.728
Authors: John Ferrin; Sreenatha Kirakodu; David Jensen; Ahmad Al-Attar; Rebecca Peyyala; M John Novak; Dolph Dawson; Mohanad Al-Sabbagh; Arnold J Stromberg; Luis Orraca; Janis Gonzalez-Martinez; Armando Burgos; Jeffrey L Ebersole; Octavio A Gonzalez Journal: J Periodontol Date: 2018-07-20 Impact factor: 6.993
Authors: Jeffrey L Ebersole; Luis Orraca; Terry B Kensler; Janis Gonzalez-Martinez; Elisabeth Maldonado; Octavio A Gonzalez Journal: J Periodontal Res Date: 2018-09-11 Impact factor: 4.419