Boyi Wang1, Jun Jiang2, Zhongcai Fan1, Rui Jiang3, Run Wang4, Haocheng Lin4. 1. Department of Cardiovascular Diseases, Affiliated Hospital, Luzhou Medical College, Luzhou, China. 2. Department of Vascular Surgery, Affiliated Hospital, Luzhou Medical College, Luzhou, China. 3. Department of Urology, Affiliated Hospital, Luzhou Medical College, Luzhou, China. Electronic address: jiangrui@126.com. 4. Department of Surgery, The University of Texas Medical School at Houston, Houston, TX.
Abstract
OBJECTIVE: To investigate the expression of sphingosine 1-phosphate 1-3 (S1P1-3) on penile cavernous tissues in hypertensive and normotensive rats and its association with erectile function. METHODS: Ten 14-week-old male spontaneously hypertensive rats (SHRs) and 10 male age-matched normotensive Wistar-Kyoto rats were randomly designated as hypertensive rat group and normotensive control group, respectively. Mean blood pressure, serum testosterone, and maximum intracavernosal pressure/mean arterial pressure (ICPmax-to-MAP ratio) were measured. The expression of S1P1-3, endothelial nitric oxide synthase (eNOS), phosphorylated eNOS (P-eNOS), and rho-associated protein kinase 1-2 (ROCK1-2) was determined in the cavernous tissues of SHRs and Wistar-Kyoto rats by the immunohistochemical study and Western blot. RESULTS: There was no significant difference in body weight and serum testosterone levels between the 2 groups. The MAP in the hypertensive rat group was significantly increased compared with the control group. After 3- and 5-V electrostimulation, the ICPmax-to-MAP ratio in the control group was significantly increased compared with the hypertensive rat group. The protein expression of eNOS, P-eNOS, and S1P1 was significantly higher in the control group than in the hypertensive rat group (P <.05). The protein expression of S1P2, S1P3, and ROCK1-2 was significantly lower in the normotensive control group than in the hypertensive rat group (P <.05). CONCLUSION: Hypertension may impair the erectile function by means of downregulating the expression of S1P1 and upregulating the expression of S1P2-3 in cavernous tissues of SHRs.
OBJECTIVE: To investigate the expression of sphingosine 1-phosphate 1-3 (S1P1-3) on penile cavernous tissues in hypertensive and normotensive rats and its association with erectile function. METHODS: Ten 14-week-old male spontaneously hypertensiverats (SHRs) and 10 male age-matched normotensive Wistar-Kyoto rats were randomly designated as hypertensiverat group and normotensive control group, respectively. Mean blood pressure, serum testosterone, and maximum intracavernosal pressure/mean arterial pressure (ICPmax-to-MAP ratio) were measured. The expression of S1P1-3, endothelial nitric oxide synthase (eNOS), phosphorylated eNOS (P-eNOS), and rho-associated protein kinase 1-2 (ROCK1-2) was determined in the cavernous tissues of SHRs and Wistar-Kyoto rats by the immunohistochemical study and Western blot. RESULTS: There was no significant difference in body weight and serum testosterone levels between the 2 groups. The MAP in the hypertensiverat group was significantly increased compared with the control group. After 3- and 5-V electrostimulation, the ICPmax-to-MAP ratio in the control group was significantly increased compared with the hypertensiverat group. The protein expression of eNOS, P-eNOS, and S1P1 was significantly higher in the control group than in the hypertensiverat group (P <.05). The protein expression of S1P2, S1P3, and ROCK1-2 was significantly lower in the normotensive control group than in the hypertensiverat group (P <.05). CONCLUSION:Hypertension may impair the erectile function by means of downregulating the expression of S1P1 and upregulating the expression of S1P2-3 in cavernous tissues of SHRs.