I Latorre1, J Díaz1, I Mialdea2, M Serra-Vidal1, N Altet3, C Prat1, N Díez2, A Escribano2, I Casas4, C Rodrigo5, V Ausina1, M Ruhwald6, J Domínguez7. 1. Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Badalona, Spain; Universitat Autònoma de Barcelona, Bellaterra, Spain; CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III, Badalona, Spain. 2. Unidad de Neumología Infantil, Hospital Clínico Universitario Valencia, Universidad de Valencia, Spain. 3. Unidad de Prevención y Control de la Tuberculosis de Barcelona, Spain; Universitat Autònoma de Barcelona, Bellaterra, Spain. 4. Servei de Medicina Preventiva, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Badalona, Spain; Universitat Autònoma de Barcelona, Bellaterra, Spain. 5. Universitat Autònoma de Barcelona, Bellaterra, Spain; Servei de Pediatria, Hospital Universitari Germans Trias I Pujol, Institut d'Investigació Germans Trias i Pujol, Badalona, Spain. 6. Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark. 7. Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Badalona, Spain; Universitat Autònoma de Barcelona, Bellaterra, Spain; CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III, Badalona, Spain. Electronic address: jadomb@gmail.com.
Abstract
OBJECTIVE: Performance of IFN-γ assays in children is compromised. Therefore, we investigated the utility of IP-10 for the detection of active tuberculosis (TB) and latent tuberculosis infection (LTBI) diagnosis in children; comparing its positivity with QuantiFERON-TB Gold In-Tube (QFN-G-IT) and T-SPOT.TB. METHODS: We studied 230 children from three groups: active TB, screening (healthy children without known exposure to active TB patient screened at school or by their paediatrician) and contact-tracing studies. IFN-γ release was determined by QFN-G-IT and T-SPOT.TB. IP-10 was detected in QFN-G-IT supernatants by ELISA. RESULTS: When combining QFN-G-IT and IP-10 assays, positive results improved significantly from 38.3% in QFN-G-IT and 33.9% in IP-10 to 41.3%. Age and type of contact were significant risk factors associated with positive QFN-G-IT and IP-10 results. IP-10 levels after antigen-specific stimulation were significantly higher in comparison to IFN-γ levels. Correlation between the three assays was good (κ = 0.717-0.783). CONCLUSIONS: IP-10 cytokine is expressed in response to TB specific-antigens used in QFN-G-IT. In conclusion, the use of IFN-γ T-cell based assays in combination with an additional IP-10 assay detection could be useful for diagnosing active TB and LTBI in children.
OBJECTIVE: Performance of IFN-γ assays in children is compromised. Therefore, we investigated the utility of IP-10 for the detection of active tuberculosis (TB) and latent tuberculosis infection (LTBI) diagnosis in children; comparing its positivity with QuantiFERON-TB Gold In-Tube (QFN-G-IT) and T-SPOT.TB. METHODS: We studied 230 children from three groups: active TB, screening (healthy children without known exposure to active TB patient screened at school or by their paediatrician) and contact-tracing studies. IFN-γ release was determined by QFN-G-IT and T-SPOT.TB. IP-10 was detected in QFN-G-IT supernatants by ELISA. RESULTS: When combining QFN-G-IT and IP-10 assays, positive results improved significantly from 38.3% in QFN-G-IT and 33.9% in IP-10 to 41.3%. Age and type of contact were significant risk factors associated with positive QFN-G-IT and IP-10 results. IP-10 levels after antigen-specific stimulation were significantly higher in comparison to IFN-γ levels. Correlation between the three assays was good (κ = 0.717-0.783). CONCLUSIONS:IP-10 cytokine is expressed in response to TB specific-antigens used in QFN-G-IT. In conclusion, the use of IFN-γ T-cell based assays in combination with an additional IP-10 assay detection could be useful for diagnosing active TB and LTBI in children.
Authors: Thomas Blauenfeldt; Raquel Villar-Hernández; Esther García-García; Irene Latorre; Line Lindebo Holm; Beatriz Muriel-Moreno; Maria Luiza De Souza-Galvão; Joan Pau Millet; Fina Sabriá; Adrián Sánchez-Montalva; Juan Ruiz-Manzano; Jose Pilarte; María A Jiménez; Carmen Centeno; Carmen Torres; Israel Molina-Pinargote; Yoel D González-Díaz; Javier Santiago; Adela Cantos; Cristina Prat; Peter Andersen; Jose Domínguez; Morten Ruhwald Journal: J Clin Microbiol Date: 2020-09-22 Impact factor: 5.948
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Authors: R Villar-Hernández; I Latorre; M L De Souza-Galvão; M A Jiménez; J Ruiz-Manzano; J Pilarte; E García-García; B Muriel-Moreno; A Cantos; N Altet; J P Millet; Y González-Díaz; I Molina-Pinargote; C Prat; M Ruhwald; J Domínguez Journal: Sci Rep Date: 2019-03-08 Impact factor: 4.379