Literature DB >> 24974041

Ratiometric imaging of pH probes.

Bree K Grillo-Hill1, Bradley A Webb1, Diane L Barber1.   

Abstract

Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical considerations of various ratiometric pH-sensitive probes including dyes and genetically encoded sensors. These probes can be used to measure pH across physical scales from macroscopic whole-mount tissues down to organelles and subcellular domains. We describe protocols for loading pH-sensitive probes into single cells or tissues and discuss ratiometric image acquisition and analysis.
© 2014 Elsevier Inc. All rights reserved.

Keywords:  Drosophila melanogaster; Genetically encoded pH biosensor; In situ imaging; Live-cell imaging; Quantitative imaging; Ratiometric imaging; Whole-mount tissue imaging; pH measurement; pH-sensitive dyes

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Year:  2014        PMID： 24974041     DOI： 10.1016/B978-0-12-420138-5.00023-9

Source DB:  PubMed          Journal:  Methods Cell Biol        ISSN： 0091-679X            Impact factor:   1.441


  19 in total

1.  An acidic residue buried in the dimer interface of isocitrate dehydrogenase 1 (IDH1) helps regulate catalysis and pH sensitivity.

Authors:  Lucas A Luna; Zachary Lesecq; Katharine A White; An Hoang; David A Scott; Olga Zagnitko; Andrey A Bobkov; Diane L Barber; Jamie M Schiffer; Daniel G Isom; Christal D Sohl
Journal:  Biochem J       Date:  2020-08-28       Impact factor: 3.857

2.  Acid-Induced Downregulation of ASS1 Contributes to the Maintenance of Intracellular pH in Cancer.

Authors:  Omer Goldman; Odeya Boukobza Assayag; Alon Silberman; Adi Jacob; Shiran Rabinovich; Lital Adler; Joo Sang Lee; Rom Keshet; Alona Sarver; Julia Frug; Noa Stettner; Sivan Galai; Erez Persi; Keren Bahar Halpern; Yehudit Zaltsman-Amir; Ben Pode-Shakked; Raya Eilam; Yair Anikster; Sandesh C S Nagamani; Igor Ulitsky; Eytan Ruppin; Ayelet Erez
Journal:  Cancer Res       Date:  2018-12-20       Impact factor: 12.701

3.  Intracellular pH controls WNT downstream of glycolysis in amniote embryos.

Authors:  Masayuki Oginuma; Yukiko Harima; Oscar A Tarazona; Margarete Diaz-Cuadros; Arthur Michaut; Tohru Ishitani; Fengzhu Xiong; Olivier Pourquié
Journal:  Nature       Date:  2020-06-24       Impact factor: 49.962

4.  Ratiometric fluorescent pH-sensitive polymers for high-throughput monitoring of extracellular pH.

Authors:  Liqiang Zhang; Fengyu Su; Xiangxing Kong; Fred Lee; Kevin Day; Weimin Gao; Mary E Vecera; Jeremy M Sohr; Sean Buizer; Yanqing Tian; Deirdre R Meldrum
Journal:  RSC Adv       Date:  2016-05-03       Impact factor: 3.361

Review 5.  pH biosensing in the plant apoplast-a focus on root cell elongation.

Authors:  Hortense Moreau; Sabine D Zimmermann; Isabelle Gaillard; Nadine Paris
Journal:  Plant Physiol       Date:  2021-10-05       Impact factor: 8.005

6.  Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue.

Authors:  Sumitra Tatapudy; Marimar Benitez; Todd Nystul
Journal:  J Vis Exp       Date:  2017-09-26       Impact factor: 1.355

7.  pHLARE: a new biosensor reveals decreased lysosome pH in cancer cells.

Authors:  Bradley A Webb; Francesca M Aloisio; Rabab A Charafeddine; Jessica Cook; Torsten Wittmann; Diane L Barber
Journal:  Mol Biol Cell       Date:  2020-11-25       Impact factor: 4.138

8.  Ethyl isopropyl amiloride decreases oxidative phosphorylation and increases mitochondrial fusion in clonal untransformed and cancer cells.

Authors:  Sagar S Manoli; Kyle Kisor; Bradley A Webb; Diane L Barber
Journal:  Am J Physiol Cell Physiol       Date:  2021-05-26       Impact factor: 5.282

9.  Genetically Encoded, pH-Sensitive mTFP1 Biosensor for Probing Lysosomal pH.

Authors:  Marcus Y Chin; Anand R Patwardhan; Kean-Hooi Ang; Austin L Wang; Carolina Alquezar; Mackenzie Welch; Phi T Nguyen; Michael Grabe; Anna V Molofsky; Michelle R Arkin; Aimee W Kao
Journal:  ACS Sens       Date:  2021-06-08       Impact factor: 7.711

10.  Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.

Authors:  Amy H Ponsford; Thomas A Ryan; Andrea Raimondi; Emanuele Cocucci; Susanne A Wycislo; Florian Fröhlich; Laura E Swan; Massimiliano Stagi
Journal:  Autophagy       Date:  2020-06-09       Impact factor: 16.016
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