Jogi Madhuprakash1, Karunakar Tanneeru2, Bhavana Karlapudi1, Lalitha Guruprasad3, Appa Rao Podile4. 1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad 500046, A.P., India. 2. School of Chemistry, University of Hyderabad, Gachibowli, Hyderabad 500046, A.P., India. 3. School of Chemistry, University of Hyderabad, Gachibowli, Hyderabad 500046, A.P., India. Electronic address: lgpsc@uohyd.ernet.in. 4. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad 500046, A.P., India. Electronic address: arpsl@uohyd.ernet.in.
Abstract
BACKGROUND: Transglycosylation (TG) activity is a property of glycosyl hydrolases (GHs) with which new glycosidic bonds are introduced between donor and acceptor sugar molecules. This special property of the GHs has potential to generate longer chain chitooligosaccharides (CHOS) that show elicitor activity in plants. We hypothesize that TG activity could be improved by retaining the substrate for a longer duration in the catalytic site. METHODS: Four variants of chitinase D from Serratia proteamaculans (SpChiD) i.e. G119S, G119W, W120A and G201W were analyzed in detail for improved TG activity using high performance liquid chromatography (HPLC) and high resolution mass spectrometry (HRMS). The results were strongly supported by 50ns molecular dynamics (MD) simulations and estimated solvated interaction energies (SIE). RESULTS: The mutant G119W lost much of both hydrolytic and TG activities, while the mutant G201W displayed increased TG. The trajectory of MD simulations of the mutant G119W showed that the indole rings of two adjacent Trp residues create a major hindrance for the DP4 movement towards the catalytic center. Increased van der Waals (vdW) and coulombic interactions between DP4 substrate and the Trp-201 resulted in enhanced TG activity with the mutant G201W. The average number of hydrogen bonds observed for the DP4 substrate was increased for the mutants G119W and G201W compared to SpChiD. CONCLUSION: The increase in TG activity could be due to partial blocking of product exit of SpChiD. GENERAL SIGNIFICANCE: This new approach can be used for generating mutants of GHs with improved TG activity to produce longer chain oligosaccharides.
BACKGROUND: Transglycosylation (TG) activity is a property of glycosyl hydrolases (GHs) with which new glycosidic bonds are introduced between donor and acceptor sugar molecules. This special property of the GHs has potential to generate longer chain chitooligosaccharides (CHOS) that show elicitor activity in plants. We hypothesize that TG activity could be improved by retaining the substrate for a longer duration in the catalytic site. METHODS: Four variants of chitinase D from Serratia proteamaculans (SpChiD) i.e. G119S, G119W, W120A and G201W were analyzed in detail for improved TG activity using high performance liquid chromatography (HPLC) and high resolution mass spectrometry (HRMS). The results were strongly supported by 50ns molecular dynamics (MD) simulations and estimated solvated interaction energies (SIE). RESULTS: The mutant G119W lost much of both hydrolytic and TG activities, while the mutant G201W displayed increased TG. The trajectory of MD simulations of the mutant G119W showed that the indole rings of two adjacent Trp residues create a major hindrance for the DP4 movement towards the catalytic center. Increased van der Waals (vdW) and coulombic interactions between DP4 substrate and the Trp-201 resulted in enhanced TG activity with the mutant G201W. The average number of hydrogen bonds observed for the DP4 substrate was increased for the mutants G119W and G201W compared to SpChiD. CONCLUSION: The increase in TG activity could be due to partial blocking of product exit of SpChiD. GENERAL SIGNIFICANCE: This new approach can be used for generating mutants of GHs with improved TG activity to produce longer chain oligosaccharides.