Effects of spatial smoothing on inter-subject correlation based analysis of FMRI.

This study evaluates the effects of spatial smoothing on inter-subject correlation (ISC) analysis for FMRI data using the traditional model based analysis as a reference. So far within ISC analysis the effects of smoothing have not been studied systematically and linear Gaussian filters with varying kernel widths have been used without better knowledge about the effects of filtering. Instead, with the traditional general linear model (GLM) based analysis, the effects of smoothing have been studied extensively. In this study, ISC and GLM analyses were computed with two experimental and one simulated block-design datasets. The test statistics and the detected activation areas were compared numerically with correlation and Dice similarity measures, respectively. The study verified that (1) the choice of the filter substantially affected the activations detected by ISC analysis, (2) the detected activations according to ISC and GLM methods were highly similar regardless of the smoothing kernel and (3) the effect of spatial smoothing was mildly smaller on ISC than GLM analysis. Our results indicated that a good selection of the full width at half maximum of the Gaussian smoothing kernel for ISC was slightly larger than double the original voxel size.