Minhua Ye1, Penghui Ye, Weizhu Zhang, Jiaqi Rao, Zijun Xie. 1. Guangdong General Hospital, Guangdong Academy of Medical Sciences, Concord Medical Center, Guangzhou 510080, China.E-mail: minhuayip2014 @outlook.com.
Abstract
OBJECTIVE: To evaluate the diagnostic value of salivary and plasma miR-21 in patients with esophageal cancer (EC). METHODS: Total RNA was extracted from saliva and plasma samples from 50 stage I and 50 stage II patients with EC and 50 healthy controls for measurement of miR-21 levels using qPCR. The diagnostic values of salivary and plasma miR-21 levels were assessed for stage I, stage II, and stage I+II EC. RESULTS: Salivary and plasma miR-21 were significantly higher in the EC patients than in the control group. The diagnostic sensitivities of plasma miR-21 for stage I, stage II, and stage I+II EC were 96%, 64% and 97%, with specificities of 44%, 84%, and 56%, respectively; the sensitivities of salivary miR-21 were 90%, 88%, and 89%, respectively, with the same specificities of 64%. Regardless of EC staging, the expression of plasma miR-21 showed a significant positive correlation with that of salivary miR-21, and their diagnostic values were comparable. CONCLUSION: Both salivary and plasmatic miR-21 can be sensitive biomarkers for EC, and salivary miR-21 detection has the potential to replace plasma detection for EC diagnosis.
OBJECTIVE: To evaluate the diagnostic value of salivary and plasma miR-21 in patients with esophageal cancer (EC). METHODS: Total RNA was extracted from saliva and plasma samples from 50 stage I and 50 stage II patients with EC and 50 healthy controls for measurement of miR-21 levels using qPCR. The diagnostic values of salivary and plasma miR-21 levels were assessed for stage I, stage II, and stage I+II EC. RESULTS: Salivary and plasma miR-21 were significantly higher in the EC patients than in the control group. The diagnostic sensitivities of plasma miR-21 for stage I, stage II, and stage I+II EC were 96%, 64% and 97%, with specificities of 44%, 84%, and 56%, respectively; the sensitivities of salivary miR-21 were 90%, 88%, and 89%, respectively, with the same specificities of 64%. Regardless of EC staging, the expression of plasma miR-21 showed a significant positive correlation with that of salivary miR-21, and their diagnostic values were comparable. CONCLUSION: Both salivary and plasmatic miR-21 can be sensitive biomarkers for EC, and salivary miR-21 detection has the potential to replace plasma detection for EC diagnosis.
Authors: Óscar Rapado-González; Cristina Martínez-Reglero; Ángel Salgado-Barreira; Bahi Takkouche; Rafael López-López; María Mercedes Suárez-Cunqueiro; Laura Muinelo-Romay Journal: Ann Med Date: 2020-04-11 Impact factor: 4.709