Chiaw Ling Chng1, Oi Fah Lai2, Charmaine Sze-Min Chew2, Yu Pei Peh2, Stephanie Man-Chung Fook-Chong2, Lay Leng Seah3, Daphne Hsu-Chin Khoo1. 1. Department of Endocrinology, Singapore General Hospital, Level 3, the Academia, 20 College Road 169856, Singapore. 2. Department of Clinical Research, Academia Discovery Tower Level 9 Research Office 2, 20 College Road 169856, Singapore. 3. Oculoplastic/Aesthetic Eyeplastic Service, Singapore National Eye Centre, 11 Third Hospital Avenue 168751, Singapore.
Abstract
AIM: To assess the effects of hypoxia on human orbital fibroblasts (OF) on adipogenesis and adipocytokine production. METHODS: Human OF were derived from tissues obtained from patients with Graves' ophthalmopathy (GO) and from patients without known thyroid diseases undergoing blepharoplasty. The OF were cultured separately under normoxic and hypoxic conditions. Comparisons of adipocytokine concentrations using multiplex ELISA and lipid accumulation in the cells using Oil Red O staining were subsequently performed. RESULTS: There was increased adipogenesis in OF from GO subject when exposed to hypoxic culture conditions. This was not observed in OF from normal controls. Hypoxia led to an increase in leptin and a decrease in MCP-1 secretion in OF cultures. CONCLUSION: Hypoxia induces adipogenesis in OF and may represent a mechanism by which smoking contributes to deterioration of GO. We also found novel changes to leptin and MCP-1 production in OF cultures exposed to hypoxia suggesting important roles of these cytokines in the disease process.
AIM: To assess the effects of hypoxia on human orbital fibroblasts (OF) on adipogenesis and adipocytokine production. METHODS:Human OF were derived from tissues obtained from patients with Graves' ophthalmopathy (GO) and from patients without known thyroid diseases undergoing blepharoplasty. The OF were cultured separately under normoxic and hypoxic conditions. Comparisons of adipocytokine concentrations using multiplex ELISA and lipid accumulation in the cells using Oil Red O staining were subsequently performed. RESULTS: There was increased adipogenesis in OF from GO subject when exposed to hypoxic culture conditions. This was not observed in OF from normal controls. Hypoxia led to an increase in leptin and a decrease in MCP-1 secretion in OF cultures. CONCLUSION:Hypoxia induces adipogenesis in OF and may represent a mechanism by which smoking contributes to deterioration of GO. We also found novel changes to leptin and MCP-1 production in OF cultures exposed to hypoxia suggesting important roles of these cytokines in the disease process.
Authors: Birgit Gustafson; Ann Hammarstedt; Christian X Andersson; Ulf Smith Journal: Arterioscler Thromb Vasc Biol Date: 2007-09-06 Impact factor: 8.311
Authors: R W Valyasevi; D Z Erickson; D A Harteneck; C M Dutton; A E Heufelder; S C Jyonouchi; R S Bahn Journal: J Clin Endocrinol Metab Date: 1999-07 Impact factor: 5.958
Authors: M R Sierra-Honigmann; A K Nath; C Murakami; G García-Cardeña; A Papapetropoulos; W C Sessa; L A Madge; J S Schechner; M B Schwabb; P J Polverini; J R Flores-Riveros Journal: Science Date: 1998-09-11 Impact factor: 47.728
Authors: Hamid Zarkesh-Esfahani; Alan G Pockley; Zida Wu; Paul G Hellewell; Anthony P Weetman; Richard J M Ross Journal: J Immunol Date: 2004-02-01 Impact factor: 5.422
Authors: A Sadeghi-Tari; M Jamshidian-Tehrani; A Nabavi; S Sharif-Kashani; E Elhami; N Hassanpour; K Ameli Zamani Journal: Eye (Lond) Date: 2016-08-19 Impact factor: 3.775