Li Jin1, Daliang Yu1, Xiaomei Li1, Ning Yu1, Xiangpei Li1, Yuanmin Wang2, Yiping Wang3. 1. Department of Rheumatology and Immunology, Anhui Provincial Hospital Affiliated to Anhui Medical University Hefei, Anhui 230001, China. 2. Centre for Kidney Research, Children's Hospital at Westmead, Westmead, The University of Sydney NSW 2145, Australia. 3. Centres for Transplantation and Renal Research, Westmead Millennium Institute, The University of Sydney NSW 2145, Australia.
Abstract
OBJECTIVE: To examine amount of CD4+CXCR5+Tfh cells and B cells subsets in salivary gland and peripheral blood from patients with primary Sjogren's syndrome (pSS) and to analyze whether the frequency of CD4+CXCR5+Tfh cells is associated with pSS pathologic process. METHODS: The percentages of CD4+CXCR5+Tfh cells and B cell subsets were examined by flow cytometry. B-lymphocyte chemoattraetant (BLC; also called CXCL13), IL-21, IL-6 from the serum of pSS patients was assessed by polymerase chain reaction-enzyme-linked immunosorbent assay (ELISA). RESULTS: The percentages of CD4+CXCR5+Tfh cells in peripheral blood were increased in pSS patients, but decreased after treatment with glucocorticoid and/or immunosuppressive drugs. Abnormal B cell subsets appeared in salivary and peripheral blood of pSS patients. The frequency of salivary CD4+CXCR5+Tfh cells was positively correlated with CD19+CD27+ memory B cells and CD19+CD27high plasma cells. Also increase of salivary CD19+CD27high plasma cells was positively associated with serum ANA titer of pSS patients. CONCLUSIONS: CD4+CXCR5+Tfh cells are significantly increased in salivary and peripheral blood in pSS patients with aberrant CD19+CD27+ memory B cells and CD19+CD27high plasma cells, suggesting that CD4+CXCR5+Tfh cells may contribute to the pathogenesis of pSS by promoting the maturation of B cells.
OBJECTIVE: To examine amount of CD4+CXCR5+Tfh cells and B cells subsets in salivary gland and peripheral blood from patients with primary Sjogren's syndrome (pSS) and to analyze whether the frequency of CD4+CXCR5+Tfh cells is associated with pSS pathologic process. METHODS: The percentages of CD4+CXCR5+Tfh cells and B cell subsets were examined by flow cytometry. B-lymphocyte chemoattraetant (BLC; also called CXCL13), IL-21, IL-6 from the serum of pSSpatients was assessed by polymerase chain reaction-enzyme-linked immunosorbent assay (ELISA). RESULTS: The percentages of CD4+CXCR5+Tfh cells in peripheral blood were increased in pSSpatients, but decreased after treatment with glucocorticoid and/or immunosuppressive drugs. Abnormal B cell subsets appeared in salivary and peripheral blood of pSSpatients. The frequency of salivary CD4+CXCR5+Tfh cells was positively correlated with CD19+CD27+ memory B cells and CD19+CD27high plasma cells. Also increase of salivary CD19+CD27high plasma cells was positively associated with serum ANA titer of pSSpatients. CONCLUSIONS:CD4+CXCR5+Tfh cells are significantly increased in salivary and peripheral blood in pSSpatients with aberrant CD19+CD27+ memory B cells and CD19+CD27high plasma cells, suggesting that CD4+CXCR5+Tfh cells may contribute to the pathogenesis of pSS by promoting the maturation of B cells.
Entities:
Keywords:
B cell; Sjogren’s syndrome; follicular helper T cell; germinal center
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