Eun-Hyeong Shim1, Se-Young Choung. 1. Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Seoul, Korea.
Abstract
OBJECTIVES: We aimed to investigate the inhibitory efficacy of Solanum tuberosum L. var. Vitelotte (SV) extract on atopic dermatitis (AD)-like skin lesions induced by the topical application of 2,4-dinitrochlorobenzene in NC/Nga mice. METHODS: SV extract was administered orally to NC/Nga mice at the dose of 75, 150 or 300 mg/kg for 4 weeks. The effectiveness of SV extract in NC/Nga mice was evaluated by measuring symptom severity, ear thickness, scratching behaviour, serum levels of IgE, IgG1 and IgG2a, T helper 1 (Th1; interferon-γ and IL-12) and Th2 cytokines (IL-4 and IL-13) in spleen, messenger RNA (mRNA) expression of inflammatory cytokines and chemokines in tissue and infiltration of inflammatory cells in tissue. KEY FINDINGS: Oral administration of SV extract to NC/Nga mice resulted in the inhibition of the development of AD-like skin lesions. SV extract was attenuated AD-like skin lesion, ear thickening and scratching behaviour. SV extract also alleviated infiltrated inflammatory cells in tissue. Production of Th1 and Th2 cytokines was inhibited in splenocyte cultures. Additionally, reduced levels of IgE and IgG1/IgG2a ratio in serum and expression of AD-related mRNAs in lesional skins were observed in SV-treated mice compared with control group. CONCLUSIONS: SV extract alleviated the exacerbation of AD-like skin lesions in NC/Nga mice by suppressing total serum level of IgE and correcting the Th1/Th2 balance.
OBJECTIVES: We aimed to investigate the inhibitory efficacy of Solanum tuberosum L. var. Vitelotte (SV) extract on atopic dermatitis (AD)-like skin lesions induced by the topical application of 2,4-dinitrochlorobenzene in NC/Ngamice. METHODS: SV extract was administered orally to NC/Ngamice at the dose of 75, 150 or 300 mg/kg for 4 weeks. The effectiveness of SV extract in NC/Ngamice was evaluated by measuring symptom severity, ear thickness, scratching behaviour, serum levels of IgE, IgG1 and IgG2a, T helper 1 (Th1; interferon-γ and IL-12) and Th2 cytokines (IL-4 and IL-13) in spleen, messenger RNA (mRNA) expression of inflammatory cytokines and chemokines in tissue and infiltration of inflammatory cells in tissue. KEY FINDINGS: Oral administration of SV extract to NC/Ngamice resulted in the inhibition of the development of AD-like skin lesions. SV extract was attenuated AD-like skin lesion, ear thickening and scratching behaviour. SV extract also alleviated infiltrated inflammatory cells in tissue. Production of Th1 and Th2 cytokines was inhibited in splenocyte cultures. Additionally, reduced levels of IgE and IgG1/IgG2a ratio in serum and expression of AD-related mRNAs in lesional skins were observed in SV-treated mice compared with control group. CONCLUSIONS: SV extract alleviated the exacerbation of AD-like skin lesions in NC/Ngamice by suppressing total serum level of IgE and correcting the Th1/Th2 balance.