| Literature DB >> 2496117 |
Abstract
The pE194 replication origin was localized to a 265-base-pair interval by analyzing the ability of purified pE194 restriction fragments to direct replication of heterologous plasmids. Replication was dependent upon RepF protein supplied in trans. The origin region contained a GC-rich dyad symmetry which may serve as the RepF target.Entities:
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Year: 1989 PMID: 2496117 PMCID: PMC209975 DOI: 10.1128/jb.171.5.2866-2869.1989
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490