Ravi Kumar Gudipaneni1, Vijay Kumar R2, Jesudass G3, Suresh Peddengatagari4, Yesuratnam Duddu5. 1. Assistant Professor, Department of Pedodontics and Preventive Dentistry, Government Dental College and Hospital , Kadapa, Andhra Pradesh, India . 2. Senior Lecturer, Department of Periodontics, Government Dental College and Hospital , Kadapa, Andhra Pradesh, India . 3. Associate Professor, Department of Pedodontics and Preventive Dentistry, Government Dental College and Hospital , Kadapa, Andhra Pradesh, India . 4. Associate Professor, Department of Periodontics, Government Dental College and Hospital , Kadapa, Andhra Pradesh, India . 5. Assistant Professor, Department of Oral and Maxillofacial Surgery, Government Dental College and Hospital , Kadapa, Andhra Pradesh, India .
Abstract
BACKGROUND AND OBJECTIVES: The present study is conducted to compare the anti-microbial efficacy of tooth paste containing lactoferrin, lysozyme, lactoperoxidase (BioXtra ®), a 500ppm fluoride tooth paste, and a non fluoridated tooth paste in children with Severe Early Childhood Caries (S-ECC). MATERIALS AND METHODS: Study group included 30 children with S-ECC aged 3-5 years. Subjects were randomly selected & divided into three groups of ten each. Group I: Non-fluoride tooth paste, Group II: 500 ppm fluoride tooth paste, Group III: tooth paste containing lactoferrin, lysozyme & lactoperoxidase. Estimation of salivary S. mutans and L. acidophilus levels was accomplished by collecting salivary samples and inoculated on Mitis Salivarius Bacitracin agar (MSB) and Rogosa SL media. Colony Forming Units (CFUs) were counted and the results were tabulated and subjected to statistical analysis. RESULTS: Brushing with tooth paste containing lysozyme, lactoferrin and lactoperoxidase after a week showed highly significant reduction in the CFU counts of both salivary S. mutans and L. acidophilus (p<0.001), whereas 500ppm fluoride tooth paste showed a highly significant reduction in the S. mutans levels (p<0.001), but a significant reduction in L. acidophilus count (p<0.01). Non-fluoride tooth paste showed a highly significant reduction in S. mutans (p<0.001) only, but reduction in the L. acidophilus levels was insignificant (p<0.05). CONCLUSION: The present study revealed that tooth paste containing lactoferrin, lysozyme, and lactoperoxidase was highly significant in reducing the salivary levels of mutans Streptococci and L. acidophilus in children with S-ECC.
BACKGROUND AND OBJECTIVES: The present study is conducted to compare the anti-microbial efficacy of tooth paste containing lactoferrin, lysozyme, lactoperoxidase (BioXtra ®), a 500ppm fluoride tooth paste, and a non fluoridated tooth paste in children with Severe Early Childhood Caries (S-ECC). MATERIALS AND METHODS: Study group included 30 children with S-ECC aged 3-5 years. Subjects were randomly selected & divided into three groups of ten each. Group I: Non-fluoride tooth paste, Group II: 500 ppm fluoride tooth paste, Group III: tooth paste containing lactoferrin, lysozyme & lactoperoxidase. Estimation of salivary S. mutans and L. acidophilus levels was accomplished by collecting salivary samples and inoculated on Mitis Salivarius Bacitracin agar (MSB) and Rogosa SL media. Colony Forming Units (CFUs) were counted and the results were tabulated and subjected to statistical analysis. RESULTS: Brushing with tooth paste containing lysozyme, lactoferrin and lactoperoxidase after a week showed highly significant reduction in the CFU counts of both salivary S. mutans and L. acidophilus (p<0.001), whereas 500ppm fluoride tooth paste showed a highly significant reduction in the S. mutans levels (p<0.001), but a significant reduction in L. acidophilus count (p<0.01). Non-fluoride tooth paste showed a highly significant reduction in S. mutans (p<0.001) only, but reduction in the L. acidophilus levels was insignificant (p<0.05). CONCLUSION: The present study revealed that tooth paste containing lactoferrin, lysozyme, and lactoperoxidase was highly significant in reducing the salivary levels of mutans Streptococci and L. acidophilus in children with S-ECC.
Entities:
Keywords:
Dentifrices; Early childhood caries; Lactoferrin; Lactoperoxidase; Lysozyme; Saliva
Authors: Kasper Hettinga; Hein van Valenberg; Sacco de Vries; Sjef Boeren; Toon van Hooijdonk; Johan van Arendonk; Jacques Vervoort Journal: PLoS One Date: 2011-04-27 Impact factor: 3.240