OBJECTIVE: To study the effects of polysaccharides from Bletillae Rhizoma (RBP) on the protection in rats during deep second-degree burn wound healing. METHODS: Thirty SD rats were randomly divided into blank control group, model control group and RBP group. After the establishment of rat deep second-degree burn wound, the wound was observed everyday and was recorded the healing time to calculate healing rate. Venous blood was collected at, 3, 7, 14, 21 and 28 d from burn rats via vena caudalis. Tachypleus RBP a cyte lysate (TAL) was used to assay the content of LPS in the plasma. Enzyme linked immunosorbent assay (ELISA) was employed to determine the levels of related proinflammatory cytokines such as TNF-alpha and IL-6 in the serum. Flow cytometry (FCM) was used to measure the positive percent of TLR4 in the peripheral blood at different time points. RESULTS: Compared with model control group, RBP could significantly shorten the healing time (P < 0.01), decreased LPS, TNF-alpha and IL-6 levels in the serum at 7 d after burn (P < 0.01 or P < 0.05). FCM results suggested that RBP obviously decreased the positive percent of TLR4 in the peripheral blood of burn rats at 7 d after burn (P < 0.01 or P < 0.05). CONCLUSION: BSP can promote burn wound healing,which might be associated with reduction the levels of LPS, TLR4 and related proinflammatory cytokines.
OBJECTIVE: To study the effects of polysaccharides from Bletillae Rhizoma (RBP) on the protection in rats during deep second-degree burn wound healing. METHODS: Thirty SD rats were randomly divided into blank control group, model control group and RBP group. After the establishment of rat deep second-degree burn wound, the wound was observed everyday and was recorded the healing time to calculate healing rate. Venous blood was collected at, 3, 7, 14, 21 and 28 d from burn rats via vena caudalis. Tachypleus RBP a cyte lysate (TAL) was used to assay the content of LPS in the plasma. Enzyme linked immunosorbent assay (ELISA) was employed to determine the levels of related proinflammatory cytokines such as TNF-alpha and IL-6 in the serum. Flow cytometry (FCM) was used to measure the positive percent of TLR4 in the peripheral blood at different time points. RESULTS: Compared with model control group, RBP could significantly shorten the healing time (P < 0.01), decreased LPS, TNF-alpha and IL-6 levels in the serum at 7 d after burn (P < 0.01 or P < 0.05). FCM results suggested that RBP obviously decreased the positive percent of TLR4 in the peripheral blood of burn rats at 7 d after burn (P < 0.01 or P < 0.05). CONCLUSION: BSP can promote burn wound healing,which might be associated with reduction the levels of LPS, TLR4 and related proinflammatory cytokines.