BACKGROUND: Being overweight or obese is becoming increasingly common in low- and middle-income countries. The present study aimed to examine association of eight genetic variants with obesity and to estimate the cumulative effects of these variants in Chinese children. METHODS: We conducted the case-control study in a total of 2,030 subjects. Genotyping of seven novel variants was performed with matrix-assisted laser desorption ionization time of flight mass spectrometry, while rs9939609 was genotyped with tetra-primer amplification refractory mutation system analysis. RESULTS: The association of two fat mass and obesity-associated gene (FTO) single-nucleotide polymorphisms (SNPs; rs9939609 and rs62048402) with body mass index (BMI) or obesity reached nominal significance at P < 0.05. We found a cumulative effect of five SNPs on the risk of overweight and obesity (odds ratio (OR) = 1.197, 95% confidence interval (CI) = 1.068-1.342, P = 0.002). Subjects carrying 9 or more effect alleles had a 127% increased risk of overweight and obesity (OR = 2.270, 95% CI = 1.403-3.671, P = 0.001) compared with subjects who carry 6 or fewer effect alleles. CONCLUSION: We confirmed two FTO SNPs (rs62048402 and rs9939609) had nominal significant effects on BMI or obesity. We identified the cumulative effect of five SNPs on risk of overweight and obesity. The results provided evidence for identifying genetic factors related to childhood obesity.
BACKGROUND: Being overweight or obese is becoming increasingly common in low- and middle-income countries. The present study aimed to examine association of eight genetic variants with obesity and to estimate the cumulative effects of these variants in Chinese children. METHODS: We conducted the case-control study in a total of 2,030 subjects. Genotyping of seven novel variants was performed with matrix-assisted laser desorption ionization time of flight mass spectrometry, while rs9939609 was genotyped with tetra-primer amplification refractory mutation system analysis. RESULTS: The association of two fat mass and obesity-associated gene (FTO) single-nucleotide polymorphisms (SNPs; rs9939609 and rs62048402) with body mass index (BMI) or obesity reached nominal significance at P < 0.05. We found a cumulative effect of five SNPs on the risk of overweight and obesity (odds ratio (OR) = 1.197, 95% confidence interval (CI) = 1.068-1.342, P = 0.002). Subjects carrying 9 or more effect alleles had a 127% increased risk of overweight and obesity (OR = 2.270, 95% CI = 1.403-3.671, P = 0.001) compared with subjects who carry 6 or fewer effect alleles. CONCLUSION: We confirmed two FTO SNPs (rs62048402 and rs9939609) had nominal significant effects on BMI or obesity. We identified the cumulative effect of five SNPs on risk of overweight and obesity. The results provided evidence for identifying genetic factors related to childhood obesity.
Authors: Jonathan P Bradfield; Suzanne Vogelezang; Janine F Felix; Alessandra Chesi; Øyvind Helgeland; Momoko Horikoshi; Ville Karhunen; Estelle Lowry; Diana L Cousminer; Tarunveer S Ahluwalia; Elisabeth Thiering; Eileen Tai-Hui Boh; Mohammad H Zafarmand; Natalia Vilor-Tejedor; Carol A Wang; Raimo Joro; Zhanghua Chen; William J Gauderman; Niina Pitkänen; Esteban J Parra; Lindsay Fernandez-Rhodes; Akram Alyass; Claire Monnereau; John A Curtin; Christian T Have; Shana E McCormack; Mette Hollensted; Christine Frithioff-Bøjsøe; Adan Valladares-Salgado; Jesus Peralta-Romero; Yik-Ying Teo; Marie Standl; Jaakko T Leinonen; Jens-Christian Holm; Triinu Peters; Jesus Vioque; Martine Vrijheid; Angela Simpson; Adnan Custovic; Marc Vaudel; Mickaël Canouil; Virpi Lindi; Mustafa Atalay; Mika Kähönen; Olli T Raitakari; Barbera D C van Schaik; Robert I Berkowitz; Shelley A Cole; V Saroja Voruganti; Yujie Wang; Heather M Highland; Anthony G Comuzzie; Nancy F Butte; Anne E Justice; Sheila Gahagan; Estela Blanco; Terho Lehtimäki; Timo A Lakka; Johannes Hebebrand; Amélie Bonnefond; Niels Grarup; Philippe Froguel; Leo-Pekka Lyytikäinen; Miguel Cruz; Sayuko Kobes; Robert L Hanson; Babette S Zemel; Anke Hinney; Koon K Teo; David Meyre; Kari E North; Frank D Gilliland; Hans Bisgaard; Mariona Bustamante; Klaus Bonnelykke; Craig E Pennell; Fernando Rivadeneira; André G Uitterlinden; Leslie J Baier; Tanja G M Vrijkotte; Joachim Heinrich; Thorkild I A Sørensen; Seang-Mei Saw; Oluf Pedersen; Torben Hansen; Johan Eriksson; Elisabeth Widén; Mark I McCarthy; Pål R Njølstad; Christine Power; Elina Hyppönen; Sylvain Sebert; Christopher D Brown; Marjo-Riitta Järvelin; Nicholas J Timpson; Stefan Johansson; Hakon Hakonarson; Vincent W V Jaddoe; S F A Grant Journal: Hum Mol Genet Date: 2019-10-01 Impact factor: 6.150
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Authors: Paola León-Mimila; Hugo Villamil-Ramírez; Blanca E López-Contreras; Sofía Morán-Ramos; Luis R Macias-Kauffer; Víctor Acuña-Alonzo; Blanca E Del Río-Navarro; Jorge Salmerón; Rafael Velazquez-Cruz; Teresa Villarreal-Molina; Carlos A Aguilar-Salinas; Samuel Canizales-Quinteros Journal: Nutrients Date: 2018-11-01 Impact factor: 5.717
Authors: Cézane P Reuter; Elza D de Mello; Priscila T da Silva; Tássia S Borges; Elisa I Klinger; Silvia I R Franke; Andréia R de M Valim Journal: J Obes Date: 2018-09-05