Fei Zheng1, Zushun Gong1, Shanshan Xing2, Qichong Xing3. 1. Department of Cardiology, Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan, 250014, PR China. 2. Shandong University of Traditional Chinese Medicine, Jinan, 250355, PR China. 3. Department of Cardiology, Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan, 250014, PR China. Electronic address: xingqichong@126.com.
Abstract
OBJECTIVES: Plenty of studies on animals suggest the involvement of caspase-1 in the development of atherosclerosis. The aim of this study was to investigate the changes of caspase-1 in the aorta of patients with coronary atherosclerosis and its association with atherosclerotic risk factors. METHODS: We collected button-sized aortic tissues from patients (n = 48) undergoing coronary artery bypass graft (CABG) surgery. Renal arterial tissues from 18 kidney donors without atherosclerosis were taken as control. The expression of caspase-1 was determined by immunohistochemistry, and Gensini score was used to evaluate the severity of coronary atherosclerosis. RESULTS: Caspase-1 was strongly expressed in aortas from CABG patients. Spearman correlation revealed that the aortic caspase-1 expression was significantly correlated with coronary severity scores (r = 0.656, P < 0.05). The relative level of aortic caspase-1 expression was elevated in patients who were smokers or with hypertension or diabetes. The caspase-1 expression was positively correlated with total cholesterol, low density lipoprotein cholesterol, and lipoprotein(a) (P < 0.05); but negatively correlated with high density lipoprotein cholesterol (P < 0.05). CONCLUSIONS: Our findings demonstrate a highly expressed caspase-1 in aortas from patients with coronary atherosclerosis, and that modulation of caspase-1 could be a potential target for prevention and treatment of atherosclerosis.
OBJECTIVES: Plenty of studies on animals suggest the involvement of caspase-1 in the development of atherosclerosis. The aim of this study was to investigate the changes of caspase-1 in the aorta of patients with coronary atherosclerosis and its association with atherosclerotic risk factors. METHODS: We collected button-sized aortic tissues from patients (n = 48) undergoing coronary artery bypass graft (CABG) surgery. Renal arterial tissues from 18 kidney donors without atherosclerosis were taken as control. The expression of caspase-1 was determined by immunohistochemistry, and Gensini score was used to evaluate the severity of coronary atherosclerosis. RESULTS:Caspase-1 was strongly expressed in aortas from CABG patients. Spearman correlation revealed that the aortic caspase-1 expression was significantly correlated with coronary severity scores (r = 0.656, P < 0.05). The relative level of aortic caspase-1 expression was elevated in patients who were smokers or with hypertension or diabetes. The caspase-1 expression was positively correlated with total cholesterol, low density lipoprotein cholesterol, and lipoprotein(a) (P < 0.05); but negatively correlated with high density lipoprotein cholesterol (P < 0.05). CONCLUSIONS: Our findings demonstrate a highly expressed caspase-1 in aortas from patients with coronary atherosclerosis, and that modulation of caspase-1 could be a potential target for prevention and treatment of atherosclerosis.
Authors: Mansour A Alghamdi; Hilary J Wallace; Phillip E Melton; Eric K Moses; Andrew Stevenson; Laith N Al-Eitan; Suzanne Rea; Janine M Duke; Patricia L Danielsen; Cecilia M Prêle; Fiona M Wood; Mark W Fear Journal: Biomedicines Date: 2020-06-28