Miriam A Bredella1, Pouneh K Fazeli2, Scott M Daley3, Karen K Miller2, Clifford J Rosen4, Anne Klibanski2, Martin Torriani3. 1. Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. Electronic address: mbredella@partners.org. 2. Neuroendocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. 3. Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. 4. Maine Medical Center Research Institute, Scarborough, ME, USA.
Abstract
PURPOSE: Women with anorexia nervosa (AN) have increased marrow fat despite severe depletion of body fat. Recent studies have suggested that marrow fat composition may serve as a biomarker for bone quality. The purpose of our study was to investigate marrow fat composition of the femur using proton MR spectroscopy ((1)H MRS), and the relationship between measures of marrow fat composition and BMD and body composition in women with AN and normal-weight controls. MATERIALS AND METHODS: 14 women with AN (29.5±1.9years) and 12 age-matched normal-weight controls underwent (1)H MRS to determine total marrow fat content and marrow fat composition of the femoral diaphysis and soleus intramyocellular lipids und unsaturated muscle lipids. MRI was performed to quantify abdominal fat, thigh fat and muscle areas. Lumbar spine BMD, fat and lean mass were assessed by DXA. RESULTS: Subjects with AN had higher marrow fat content (p<0.05), but similar marrow fat composition (p>0.05) compared to normal-weight controls. There was an inverse association between marrow methylene protons, an estimate of fatty acid (FA) saturated bonds, and lumbar spine BMD (r=-0.52, p=0.008) independent of %ideal body weight (%IBW). Olefinic protons at 5.3ppm, an estimate of FA unsaturated bonds, were inversely associated with body fat depots, independent of %IBW, and positively associated with soleus unsaturation (p≤0.05). CONCLUSION: Women with AN have higher total femoral marrow fat but similar composition compared to normal-weight controls. The degree of marrow FA saturation correlates inversely with BMD, suggesting that saturated lipids may have negative effects on BMD. The degree of marrow FA unsaturation correlates positively with soleus unsaturation, suggesting that marrow fat composition may be influenced by the same factors as ectopic lipid composition in muscle.
PURPOSE:Women with anorexia nervosa (AN) have increased marrow fat despite severe depletion of body fat. Recent studies have suggested that marrow fat composition may serve as a biomarker for bone quality. The purpose of our study was to investigate marrow fat composition of the femur using proton MR spectroscopy ((1)H MRS), and the relationship between measures of marrow fat composition and BMD and body composition in women with AN and normal-weight controls. MATERIALS AND METHODS: 14 women with AN (29.5±1.9years) and 12 age-matched normal-weight controls underwent (1)H MRS to determine total marrow fat content and marrow fat composition of the femoral diaphysis and soleus intramyocellular lipids und unsaturated muscle lipids. MRI was performed to quantify abdominal fat, thigh fat and muscle areas. Lumbar spine BMD, fat and lean mass were assessed by DXA. RESULTS: Subjects with AN had higher marrow fat content (p<0.05), but similar marrow fat composition (p>0.05) compared to normal-weight controls. There was an inverse association between marrow methylene protons, an estimate of fatty acid (FA) saturated bonds, and lumbar spine BMD (r=-0.52, p=0.008) independent of %ideal body weight (%IBW). Olefinic protons at 5.3ppm, an estimate of FA unsaturated bonds, were inversely associated with body fat depots, independent of %IBW, and positively associated with soleus unsaturation (p≤0.05). CONCLUSION:Women with AN have higher total femoral marrow fat but similar composition compared to normal-weight controls. The degree of marrow FA saturation correlates inversely with BMD, suggesting that saturatedlipids may have negative effects on BMD. The degree of marrow FA unsaturation correlates positively with soleus unsaturation, suggesting that marrow fat composition may be influenced by the same factors as ectopic lipid composition in muscle.
Authors: Jeffrey M Gimble; Sanjin Zvonic; Z Elizabeth Floyd; Moustapha Kassem; Mark E Nuttall Journal: J Cell Biochem Date: 2006-05-15 Impact factor: 4.429
Authors: Jong Sam Lee; Srijan K Pinnamaneni; Su Ju Eo; In Ho Cho; Jae Hwan Pyo; Chang Keun Kim; Andrew J Sinclair; Mark A Febbraio; Matthew J Watt Journal: J Appl Physiol (1985) Date: 2005-12-15
Authors: S Sendhil Velan; Nicholas Said; Christopher Durst; Stephanie Frisbee; Jefferson Frisbee; Raymond R Raylman; M Albert Thomas; Vazhaikkurichi M Rajendran; Richard G Spencer; Stephen E Alway Journal: Am J Physiol Regul Integr Comp Physiol Date: 2008-07-30 Impact factor: 3.619
Authors: Basem M Abdallah; Ming Ding; Charlotte H Jensen; Nicholas Ditzel; Allan Flyvbjerg; Thomas G Jensen; Frederik Dagnaes-Hansen; Jürg A Gasser; Moustapha Kassem Journal: Endocrinology Date: 2007-04-19 Impact factor: 4.736
Authors: Sai Polineni; Megi Resulaj; Alexander T Faje; Erinne Meenaghan; Miriam A Bredella; Mary Bouxsein; Hang Lee; Ormond A MacDougald; Anne Klibanski; Pouneh K Fazeli Journal: J Bone Miner Res Date: 2020-03-23 Impact factor: 6.741
Authors: Zachary L Sebo; Elizabeth Rendina-Ruedy; Gene P Ables; Dieter M Lindskog; Matthew S Rodeheffer; Pouneh K Fazeli; Mark C Horowitz Journal: Endocr Rev Date: 2019-10-01 Impact factor: 19.871
Authors: Kirsten Ecklund; Sridhar Vajapeyam; Robert V Mulkern; Henry A Feldman; Jennifer M O'Donnell; Amy D DiVasta; Catherine M Gordon Journal: Pediatr Radiol Date: 2017-04-22