| Literature DB >> 24952075 |
Martin Krøyer Rasmussen1, Galia Zamaratskaia2, Bo Ekstrand3.
Abstract
Due to limited availability of human liver tissue for the study of cytochrome P450 (CYP450), porcine liver tissue has been suggested as an alternative source to prepare microsomes and hepatocytes. The porcine liver is made by four different lobes. The present study investigated the expression and activity of specific CYP450 isoforms in the four lobes, with the purpose to examine if one lobe of the porcine liver resembles the human more than others. Samples from the four major lobes were taken from female pigs and mRNA expression and activity of CYP1A, 2A, 2C, 2D, 2E and 3A determined. The results showed no differences in specific mRNA expression and activity of any of the investigated CYP450 isoforms. In conclusion, the study shows that all parts of the porcine liver are equally useful as model tissue.Entities:
Keywords: Detoxification; Inter-individual; Intra-hepatic; Model system; PCA; Porcine liver
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Year: 2014 PMID： 24952075 DOI： 10.1016/j.tiv.2014.06.002
Source DB: PubMed Journal: Toxicol In Vitro ISSN： 0887-2333 Impact factor: 3.500