Literature DB >> 24950007

A simple optical configuration for cell tracking by dark-field microscopy.

Vlatka Antolović1, Maja Marinović2, Vedrana Filić3, Igor Weber4.   

Abstract

We describe a simple optical configuration for dark-field microscopy at low magnification, realized with the use of standard microscope components. An inherent high contrast makes this method attractive for computer-assisted tracking and counting of microorganisms. We applied this setup for dark-field microscopy to measure the speed of migrating Dictyostelium amoebae.
Copyright © 2014 Elsevier B.V. All rights reserved.

Keywords:  Annular aperture; Cell migration; Dark-field microscopy; Dictyostelium discoideum; Long-working-distance condenser; Tracking

Mesh：

Year:  2014        PMID： 24950007     DOI： 10.1016/j.mimet.2014.06.006

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  3 in total

1.  Quantitative imaging of Rac1 activity in Dictyostelium cells with a fluorescently labelled GTPase-binding domain from DPAKa kinase.

Authors:  Maja Marinović; Marko Šoštar; Vedrana Filić; Vlatka Antolović; Igor Weber
Journal:  Histochem Cell Biol       Date:  2016-04-28       Impact factor: 4.304

2.  Dark-field microscopy enhance visibility of CD31 endothelial staining.

Authors:  Eva Jennische; Stefan Lange; Ragnar Hultborn
Journal:  Eur J Histochem       Date:  2020-07-01       Impact factor: 3.188

3.  Microscopes in Conservative Dentistry and Endodontics Research.

Authors:  Shishir Singh
Journal:  J Conserv Dent       Date:  2022-08-02
  3 in total

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