Daniele Lindemann1, Stefanie B Werle2, Daniela Steffens3, Franklin Garcia-Godoy4, Patricia Pranke5, Luciano Casagrande6. 1. Department of Pediatric Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: danilindemann@globo.com. 2. Department of Pediatric Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: stewerle@hotmail.com. 3. School of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: janine.machado@yahoo.com.br. 4. Bioscience Research Center, Senior Executive Asssociate Dean for Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee, USA. Electronic address: fgarciagodoy@gmail.com. 5. Head of Hematology and Stem Cell Laboratory and Stem Cell Research Institute, School of Pharmacy; Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: patriciapranke@ufrgs.br. 6. Department of Pediatric Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: luciano.casagrande@ufrgs.br.
Abstract
OBJECTIVES: The aim of this study was to isolate and cultivate cells from the pulp of 7-day-cryopreserved intact deciduous human teeth and evaluate the effect of cryopreservation on dental pulp stem cell (DPSC) characteristics. DESIGN: Twenty-six deciduous teeth were collected and allocated in two groups: immediate cell isolation (non-cryopreserved group) and intact cryopreserved (cryopreserved group). The teeth were cryopreserved in dimethylsulfoxide solution and recovered after 7 days. The success rate of isolation, proliferation, surface markers (CD14, CD29, CD34, CD45, CD73, CD90, and HLA-DR), differentiation capacity, and morphology were evaluated. RESULTS: Isolation success rate was 61% and 30% for the non-cryopreserved and cryopreserved groups, respectively. There were no statistical differences between the groups for the tested surface markers. The cells in both groups were capable of differentiating into three mesenchymal lineages. No statistical differences between the groups were observed through the time course proliferation assay (0, 1, 3, 5, and 7 days); however, the mean time between isolation and the fifth passage was shorter for the non-cryopreserved group (p=0.035). The morphology of the cells was considered altered in the cryopreserved group. CONCLUSION: DPSCs were obtained from cryopreserved intact deciduous teeth without changes in the immunophenotypical characteristics and differentiation ability; however, lower culture rates, proliferation potential, and morphological alterations were observed in relation to the control group.
OBJECTIVES: The aim of this study was to isolate and cultivate cells from the pulp of 7-day-cryopreserved intact deciduous human teeth and evaluate the effect of cryopreservation on dental pulp stem cell (DPSC) characteristics. DESIGN: Twenty-six deciduous teeth were collected and allocated in two groups: immediate cell isolation (non-cryopreserved group) and intact cryopreserved (cryopreserved group). The teeth were cryopreserved in dimethylsulfoxide solution and recovered after 7 days. The success rate of isolation, proliferation, surface markers (CD14, CD29, CD34, CD45, CD73, CD90, and HLA-DR), differentiation capacity, and morphology were evaluated. RESULTS: Isolation success rate was 61% and 30% for the non-cryopreserved and cryopreserved groups, respectively. There were no statistical differences between the groups for the tested surface markers. The cells in both groups were capable of differentiating into three mesenchymal lineages. No statistical differences between the groups were observed through the time course proliferation assay (0, 1, 3, 5, and 7 days); however, the mean time between isolation and the fifth passage was shorter for the non-cryopreserved group (p=0.035). The morphology of the cells was considered altered in the cryopreserved group. CONCLUSION: DPSCs were obtained from cryopreserved intact deciduous teeth without changes in the immunophenotypical characteristics and differentiation ability; however, lower culture rates, proliferation potential, and morphological alterations were observed in relation to the control group.
Authors: Gina D Kusuma; Mehri Barabadi; Jean L Tan; David A V Morton; Jessica E Frith; Rebecca Lim Journal: Front Pharmacol Date: 2018-10-29 Impact factor: 5.810
Authors: Reem S Alsulaimani; Sumaiah A Ajlan; Abdullah M Aldahmash; May S Alnabaheen; Nahid Y Ashri Journal: Saudi Med J Date: 2016-05 Impact factor: 1.484