Anna Maria Górska1, Karolina Noworyta-Sokołowska1, Krystyna Gołembiowska2. 1. Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland. 2. Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland. Electronic address: nfgolemb@cyf-kr.edu.pl.
Abstract
BACKGROUND: The psychostimulant 3,4-methylenedioxymethamphetamine (MDMA) with a strong addictive potential is widely used as a recreational drug. Neurotoxicity of MDMA is related with the generation of highly reactive free radicals. METHODS: MDMA was given in doses of 20 and 40mg/kg ip alone or in combination with caffeine (CAF) 10mg/kg ip. Extracellular concentration of hydroxyl radical was measured using microdialysis in freely moving mice and was assayed by HPLC with electrochemical detection. RESULTS: MDMA dose-dependently increased production of hydroxyl radical in the mouse striatum and its effect was reversed by caffeine. CONCLUSIONS: The data show that caffeine may have neuroprotective properties as it decreased oxidative stress induced by MDMA.
BACKGROUND: The psychostimulant 3,4-methylenedioxymethamphetamine (MDMA) with a strong addictive potential is widely used as a recreational drug. Neurotoxicity of MDMA is related with the generation of highly reactive free radicals. METHODS:MDMA was given in doses of 20 and 40mg/kg ip alone or in combination with caffeine (CAF) 10mg/kg ip. Extracellular concentration of hydroxyl radical was measured using microdialysis in freely moving mice and was assayed by HPLC with electrochemical detection. RESULTS:MDMA dose-dependently increased production of hydroxyl radical in the mouse striatum and its effect was reversed by caffeine. CONCLUSIONS: The data show that caffeine may have neuroprotective properties as it decreased oxidative stress induced by MDMA.
Authors: Anna Maria Górska; Katarzyna Kamińska; Agnieszka Wawrzczak-Bargieła; Giulia Costa; Micaela Morelli; Ryszard Przewłocki; Grzegorz Kreiner; Krystyna Gołembiowska Journal: Neurotox Res Date: 2017-11-13 Impact factor: 3.911