Alina S Porfire1, Sorin E Leucuţa2, Bela Kiss3, Felicia Loghin3, Alina E Pârvu4. 1. Department of Pharmaceutical Technology and Biopharmaceutics, University of Medicine and Pharmacy "Iuliu Hatieganu", Cluj-Napoca, Romania. Electronic address: aporfire@umfcluj.ro. 2. Department of Pharmaceutical Technology and Biopharmaceutics, University of Medicine and Pharmacy "Iuliu Hatieganu", Cluj-Napoca, Romania. 3. Department of Toxicology, University of Medicine and Pharmacy "Iuliu Hatieganu", Cluj-Napoca, Romania. 4. Department of Physiopathology, University of Medicine and Pharmacy "Iuliu Hatieganu", Cluj-Napoca, Romania.
Abstract
BACKGROUND: The current study evaluated the role of delivery system (solution, conventional liposomes and PEG-ylated liposomes) on superoxide dismutase (SOD) antioxidant and antiinflammatory properties in a rat model of lipopolysaccharide (LPS)-induced peritonitis. METHODS: Fifty male albino rats (Wistar-Bratislava) were divided into five groups (n=10). Control group received saline and the other four groups received intraperitoneal injections of LPS (5mg/kg). Among the LPS-injected groups, one was LPS control group and the other three groups received the endotoxin injection 30min after receiving the same dose of SOD (500U/kg, ip) in different delivery systems: saline solution (SOD-S), conventional liposomes (SOD-L) or PEG-ylated liposomes (SOD-PL). The animals were euthanized 6h after LPS injection, blood samples were collected and acute phase response (total and differential leukocytes count; tumor necrosis factor α), antioxidants (total antioxidants; reduced glutathione), oxidative stress (total oxidants; lipid peroxidation) and nitrosative stress (nitric oxide metabolites; nitrotyrosine) were evaluated. RESULTS: Intraperitoneal administration of LPS to rats induced a marked inflammatory and oxidative response in plasma. On the other hand, all SOD formulations had protective effect against endotoxin-induced inflammation and oxidative/nitrosative stress, but PEG-ylated liposomes had the most significant activity. Thus, SOD-PL administration significantly reduced the effects of LPS on bone marrow acute phase response, the oxidative status and production of nitric oxide metabolites, while increasing the markers of antioxidant response in a significant manner. CONCLUSION: SOD supplementation interferes both with inflammatory and oxidative pathways involved in LPS-induced acute inflammation, PEG-ylated liposomal formulation being of choice among the tested delivery systems.
BACKGROUND: The current study evaluated the role of delivery system (solution, conventional liposomes and PEG-ylated liposomes) on superoxide dismutase (SOD) antioxidant and antiinflammatory properties in a rat model of lipopolysaccharide (LPS)-induced peritonitis. METHODS: Fifty male albino rats (Wistar-Bratislava) were divided into five groups (n=10). Control group received saline and the other four groups received intraperitoneal injections of LPS (5mg/kg). Among the LPS-injected groups, one was LPS control group and the other three groups received the endotoxin injection 30min after receiving the same dose of SOD (500U/kg, ip) in different delivery systems: saline solution (SOD-S), conventional liposomes (SOD-L) or PEG-ylated liposomes (SOD-PL). The animals were euthanized 6h after LPS injection, blood samples were collected and acute phase response (total and differential leukocytes count; tumor necrosis factor α), antioxidants (total antioxidants; reduced glutathione), oxidative stress (total oxidants; lipid peroxidation) and nitrosative stress (nitric oxide metabolites; nitrotyrosine) were evaluated. RESULTS: Intraperitoneal administration of LPS to rats induced a marked inflammatory and oxidative response in plasma. On the other hand, all SOD formulations had protective effect against endotoxin-induced inflammation and oxidative/nitrosative stress, but PEG-ylated liposomes had the most significant activity. Thus, SOD-PL administration significantly reduced the effects of LPS on bone marrow acute phase response, the oxidative status and production of nitric oxide metabolites, while increasing the markers of antioxidant response in a significant manner. CONCLUSION:SOD supplementation interferes both with inflammatory and oxidative pathways involved in LPS-induced acute inflammation, PEG-ylated liposomal formulation being of choice among the tested delivery systems.
Authors: Shaheen A Farhadi; Evelyn Bracho-Sanchez; Sabrina L Freeman; Benjamin G Keselowsky; Gregory A Hudalla Journal: Bioconjug Chem Date: 2018-01-31 Impact factor: 4.774
Authors: Xuejiao Zeng; Jie Liu; Xihao Du; Jia Zhang; Kun Pan; Wei Shan; Yuquan Xie; Weimin Song; Jinzhuo Zhao Journal: Environ Sci Pollut Res Int Date: 2018-05-26 Impact factor: 4.223
Authors: Marius Emil Rusu; Carmen Georgiu; Anca Pop; Andrei Mocan; Bela Kiss; Oliviu Vostinaru; Ionel Fizesan; Maria-Georgia Stefan; Ana-Maria Gheldiu; Letitia Mates; Rebeca Moldovan; Dana Maria Muntean; Felicia Loghin; Laurian Vlase; Daniela-Saveta Popa Journal: Antioxidants (Basel) Date: 2020-05-14