Jing-jing Peng1, Biao Wu2, Xin-bo Xiao3, Yong-sheng Shao2, Yan Feng4, Meng-xin Yin4. 1. Department of Gastroenterology, General Hospital of the Yangtze River Shipping, Wuhan, Hubei Province, China. 2. Department of Gastrointestinal Surgery, The First Hospital of Wu Han City, Wuhan, Hubei Province, China. 3. Department of Gastrointestinal Surgery, The First Hospital of Wu Han City, Wuhan, Hubei Province, China. Electronic address: xiaoxbsubmission@163.com. 4. Department of Pathology, The First Hospital of Wu Han City, Wuhan, Hubei Province, China.
Abstract
BACKGROUND AND AIMS: To investigate the expression and prognostic significance of Krüppel-like factor 17 (KLF17) in human gastric cancer. METHODS: KLF17 expressions in 158 paraffin-embedded gastric cancer samples were analyzed using immunohistochemistry. In addition, KLF17 expressions patterns in three fresh gastric cancer tissues and noncancerous gastric mucosa were examined by Western blotting. The correlation between KLF17 expression and clinicopathological factors as well as patient survival was investigated. RESULTS: Immunohistochemical staining data indicated that KLF17 expression was significantly decreased in 98 of 158 gastric adenocarcinoma cases. Reduced KLF17 expression in fresh gastric cancer tissues was confirmed by Western blotting. Reduced expression of KLF17 was strongly correlated with tumor size, pN stage and lymphovascular invasion. Multivariate Cox regression analysis identified KLF17 expression as an independent prognostic factor for both overall survival (HR = 0.481, 95% CI = 0.225-0.665, p = 0.009) and disease-free survival (HR = 0.438, 95% CI = 0.254-0.758, p = 0.003). CONCLUSION: The reduced expression of KLF17 protein in gastric cancer was correlated with tumor size, pN stage and lymphovascular invasion and was an independent predictor for poor survival in patients undergoing surgery for gastric cancer.
BACKGROUND AND AIMS: To investigate the expression and prognostic significance of Krüppel-like factor 17 (KLF17) in humangastric cancer. METHODS:KLF17 expressions in 158 paraffin-embedded gastric cancer samples were analyzed using immunohistochemistry. In addition, KLF17 expressions patterns in three fresh gastric cancer tissues and noncancerous gastric mucosa were examined by Western blotting. The correlation between KLF17 expression and clinicopathological factors as well as patient survival was investigated. RESULTS: Immunohistochemical staining data indicated that KLF17 expression was significantly decreased in 98 of 158 gastric adenocarcinoma cases. Reduced KLF17 expression in fresh gastric cancer tissues was confirmed by Western blotting. Reduced expression of KLF17 was strongly correlated with tumor size, pN stage and lymphovascular invasion. Multivariate Cox regression analysis identified KLF17 expression as an independent prognostic factor for both overall survival (HR = 0.481, 95% CI = 0.225-0.665, p = 0.009) and disease-free survival (HR = 0.438, 95% CI = 0.254-0.758, p = 0.003). CONCLUSION: The reduced expression of KLF17 protein in gastric cancer was correlated with tumor size, pN stage and lymphovascular invasion and was an independent predictor for poor survival in patients undergoing surgery for gastric cancer.
Authors: Shih-Chiang Huang; Hsiao-Wei Chen; Lei Zhang; Yun-Shao Sung; Narasimhan P Agaram; Mary Davis; Morris Edelman; Christopher D M Fletcher; Cristina R Antonescu Journal: Genes Chromosomes Cancer Date: 2015-02-23 Impact factor: 5.006