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Literature DB >> 24947394

Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers.

Abstract

In oviparous animals, clathrin-dependent endocytosis is often critical to stockpile a necessary supply of yolk within the maturing oocyte, which enables subsequent embryonic development. In the physically linked chains of maturing egg chambers within the Drosophila melanogaster ovary, a distinct, morphologically discernable subset undergoes a massive burst clathrin-mediated endocytosis to accumulate yolk in a process termed vitellogenesis. Here, we describe how to prepare isolated ovaries to follow endocytosis, and detail approaches to follow live uptake of soluble reporters into vitellogenic Drosophila egg chambers.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
Clathrin

Year: 2014 PMID： 24947394 PMCID： PMC4342756 DOI： 10.1007/978-1-4939-0944-5_24

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

19 in total

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Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers.

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