In vivo and ex vivo SPME: a low invasive sampling and sample preparation tool in clinical bioanalysis.

Solid phase microextraction (SPME) is well-established technology in bioanalysis. Current review discusses the features of SPME, which determine the non- or low-invasiveness of the method in biomedical analysis. In the first section we analyze the factors, which have significant influence on the SPME sampling device performance in the view of sampling safety and efficiency. In the later sections applicability of various SPME approaches for analysis of easily accessible samples routinely used for analysis (e.g., urine, blood) as well as limited availability samples (tissues) is discussed. Moreover, the examples of sampling alternative matrices such as hair, saliva, sweat or breath are presented. The advantages and limitation of the technology in the view of future development of SPME are also reviewed.