BACKGROUND: The identification and management of hemolyzed samples are crucial issues in the development of new blood-based biomarkers. RESULTS: Using experiments of controlled hemolysis and lipemia and two plasma series from cancer patients, we developed and validated a lipemia-independent hemolysis score (HS). HS resulted strictly associated with the amount of lysed erythrocytes and with serum index measurement (reference method), highly reproducible, and able to identify as hemolyzed plasma/serum samples containing ≥6.1 mg/dl of free hemoglobin. CONCLUSION: We developed a simple, robust, sensitive, cost-effective, spectrophotometrically-based system to identify hemolyzed plasma/serum specimens. The procedure requires only 2 μl of sample, thus representing a useful tool for research studies and an essential pre-analytical quality control for an optimal biobanking of liquid biopsies.
BACKGROUND: The identification and management of hemolyzed samples are crucial issues in the development of new blood-based biomarkers. RESULTS: Using experiments of controlled hemolysis and lipemia and two plasma series from cancerpatients, we developed and validated a lipemia-independent hemolysis score (HS). HS resulted strictly associated with the amount of lysed erythrocytes and with serum index measurement (reference method), highly reproducible, and able to identify as hemolyzed plasma/serum samples containing ≥6.1 mg/dl of free hemoglobin. CONCLUSION: We developed a simple, robust, sensitive, cost-effective, spectrophotometrically-based system to identify hemolyzed plasma/serum specimens. The procedure requires only 2 μl of sample, thus representing a useful tool for research studies and an essential pre-analytical quality control for an optimal biobanking of liquid biopsies.
Authors: José Luis García-Giménez; Marta Seco-Cervera; Trygve O Tollefsbol; Carlos Romá-Mateo; Lorena Peiró-Chova; Pablo Lapunzina; Federico V Pallardó Journal: Crit Rev Clin Lab Sci Date: 2017-12-11 Impact factor: 6.250
Authors: Andrey V Zhelankin; Sergey V Vasiliev; Daria A Stonogina; Konstantin A Babalyan; Elena I Sharova; Yurii V Doludin; Dmitry Y Shchekochikhin; Eduard V Generozov; Anna S Akselrod Journal: Int J Mol Sci Date: 2020-05-15 Impact factor: 5.923
Authors: Grant C O'Connell; Connie S Tennant; Noelle Lucke-Wold; Yasser Kabbani; Abdul R Tarabishy; Paul D Chantler; Taura L Barr Journal: Sci Rep Date: 2017-10-11 Impact factor: 4.379