Wolffian duct development.

The Wolffian ducts (WDs) are the progenitors of the epididymis, vas deferens and seminal vesicles. They form initially as nephric ducts that acquire connection to the developing testis as the mesonephros regresses. The development of the WDs is dependent on androgens. Conventionally, the active androgen is believed to be testosterone delivered locally rather than via the systemic circulation. However, recent studies in marsupials show that 5α-reduced steroids are essential and that these can induce virilisation even when they are delivered via the systemic circulation. The development of the WDs involves an interplay between the duct epithelium and underlying mesenchyme; androgen receptors in both the epithelium and mesenchyme are needed. The epidermal growth factor and epidermal growth factor receptor may play a role, possibly via activation of androgen receptor. The formation of the epididymis involves a complex morphogenetic program to achieve the normal pattern of coiling, formation of septae, and regional functional differentiation. In part, this process may be mediated by inhibin beta A as well as by genes from the HOX cluster. Whilst the development of the WD is androgen dependent, it is clear that there is a complex interplay between androgens, genes and growth factors in the tissues that leads to the formation of the complex anatomy of the male reproductive duct system in the adult.