BACKGROUND: Acellular dermal matrices (ADMs) have been commonly used in expander-based breast reconstruction to provide inferolateral prosthesis coverage. Although the clinical performance of these biologic scaffold materials varies depending on a number of factors, an in-depth systematic characterization of the host response is yet to be performed. The present study evaluates the biochemical composition and structure of two ADMs, AlloDerm(®) Regenerative Tissue Matrix and AlloMax™ Surgical Graft, and provides a comprehensive spatiotemporal characterization in a porcine model of tissue expander breast reconstruction. METHODS: Each ADM was characterized with regard to thickness, permeability, donor nucleic acid content, (residual double-stranded DNA [dsDNA]), and growth factors (basic fibroblast growth factor [bFGF], vascular endothelial growth factor [VEGF], and transforming growth factor-beta 1 [TGF-β1]). Cytocompatibility was evaluated by in vitro cell culture on the ADMs. The host response was evaluated at 4 and 12 weeks at various locations within the ADMs using established metrics of the inflammatory and tissue remodeling response: cell infiltration, multinucleate giant cell formation, extent of ADM remodeling, and neovascularization. RESULTS: AlloMax incorporated more readily with surrounding host tissue as measured by earlier and greater cell infiltration, fewer foreign body giant cells, and faster remodeling of ADM. These findings correlated with the in vitro composition and cytocompatibility analysis, which showed AlloMax to more readily support in vitro cell growth. CONCLUSIONS: AlloMax and AlloDerm demonstrated distinct remodeling characteristics in a porcine model of tissue expander breast reconstruction.
BACKGROUND: Acellular dermal matrices (ADMs) have been commonly used in expander-based breast reconstruction to provide inferolateral prosthesis coverage. Although the clinical performance of these biologic scaffold materials varies depending on a number of factors, an in-depth systematic characterization of the host response is yet to be performed. The present study evaluates the biochemical composition and structure of two ADMs, AlloDerm(®) Regenerative Tissue Matrix and AlloMax™ Surgical Graft, and provides a comprehensive spatiotemporal characterization in a porcine model of tissue expander breast reconstruction. METHODS: Each ADM was characterized with regard to thickness, permeability, donor nucleic acid content, (residual double-stranded DNA [dsDNA]), and growth factors (basic fibroblast growth factor [bFGF], vascular endothelial growth factor [VEGF], and transforming growth factor-beta 1 [TGF-β1]). Cytocompatibility was evaluated by in vitro cell culture on the ADMs. The host response was evaluated at 4 and 12 weeks at various locations within the ADMs using established metrics of the inflammatory and tissue remodeling response: cell infiltration, multinucleate giant cell formation, extent of ADM remodeling, and neovascularization. RESULTS: AlloMax incorporated more readily with surrounding host tissue as measured by earlier and greater cell infiltration, fewer foreign body giant cells, and faster remodeling of ADM. These findings correlated with the in vitro composition and cytocompatibility analysis, which showed AlloMax to more readily support in vitro cell growth. CONCLUSIONS: AlloMax and AlloDerm demonstrated distinct remodeling characteristics in a porcine model of tissue expander breast reconstruction.
Authors: Bryan N Brown; John M Freund; Li Han; J Peter Rubin; Janet E Reing; Eric M Jeffries; Mathew T Wolf; Stephen Tottey; Christopher A Barnes; Buddy D Ratner; Stephen F Badylak Journal: Tissue Eng Part C Methods Date: 2011-02-05 Impact factor: 3.056
Authors: Stephen F Badylak; Jolene E Valentin; Anjani K Ravindra; George P McCabe; Ann M Stewart-Akers Journal: Tissue Eng Part A Date: 2008-11 Impact factor: 3.845
Authors: D M Faulk; C A Carruthers; H J Warner; C R Kramer; J E Reing; L Zhang; A D'Amore; S F Badylak Journal: Acta Biomater Date: 2013-09-18 Impact factor: 8.947
Authors: Adam R Kolker; Daniel J Brown; Jeremiah S Redstone; Vincent M Scarpinato; Marc K Wallack Journal: Ann Plast Surg Date: 2005-07 Impact factor: 1.539
Authors: Ekaterina Vorotnikova; Donna McIntosh; Abiche Dewilde; Jianping Zhang; Janet E Reing; Li Zhang; Kevin Cordero; Khamilia Bedelbaeva; Dimitri Gourevitch; Ellen Heber-Katz; Stephen F Badylak; Susan J Braunhut Journal: Matrix Biol Date: 2010-08-24 Impact factor: 11.583
Authors: Simon Skovsted Yde; Mette Eline Brunbjerg; Gudrun Gudmundsdottir; Mindaugas Bazys; Martin Heje; Tine Engberg Damsgaard Journal: Case Reports Plast Surg Hand Surg Date: 2017-01-16
Authors: Haitham H Khalil; Maninder Kalkat; Marco N Malahias; Saif Rhobaye; Tarek Ashour; Madava G Djearaman; Babu Naidu Journal: Plast Reconstr Surg Glob Open Date: 2018-05-18