Literature DB >> 24940791

Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

James H Felce1, Rachel G Knox2, Simon J Davis2.   

Abstract

We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membrane protein, CD86, and two G protein-coupled receptors β2AR and mCannR2, all of which behave as dimers in these conventional assays, exhibit monomeric behavior in an improved competition-based type-3 BRET assay designed to circumvent such artifacts.
Copyright © 2014 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2014        PMID： 24940791      PMCID： PMC4070276          DOI： 10.1016/j.bpj.2014.04.061

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  12 in total

1.  Opioid receptor homo- and heterodimerization in living cells by quantitative bioluminescence resonance energy transfer.

Authors:  Danxin Wang; Xiaochun Sun; Laura M Bohn; Wolfgang Sadée
Journal:  Mol Pharmacol       Date:  2005-03-18       Impact factor: 4.436

2.  Glycoprotein hormone receptors: link between receptor homodimerization and negative cooperativity.

Authors:  Eneko Urizar; Lucia Montanelli; Tiffany Loy; Marco Bonomi; Stéphane Swillens; Céline Gales; Michel Bouvier; Guillaume Smits; Gilbert Vassart; Sabine Costagliola
Journal:  EMBO J       Date:  2005-05-12       Impact factor: 11.598

3.  A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transfer.

Authors:  John R James; Marta I Oliveira; Alexandre M Carmo; Andrea Iaboni; Simon J Davis
Journal:  Nat Methods       Date:  2006-11-05       Impact factor: 28.547

Review 4.  Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET).

Authors:  Kevin D G Pfleger; Karin A Eidne
Journal:  Nat Methods       Date:  2006-03       Impact factor: 28.547

5.  Quantification of GPCR internalization by single-molecule microscopy in living cells.

Authors:  Arnauld Sergé; Sandra de Keijzer; Freek Van Hemert; Mark R Hickman; Dale Hereld; Herman P Spaink; Thomas Schmidt; B Ewa Snaar-Jagalska
Journal:  Integr Biol (Camb)       Date:  2011-05-03       Impact factor: 2.192

6.  Binding stoichiometry of the cytotoxic T lymphocyte-associated molecule-4 (CTLA-4). A disulfide-linked homodimer binds two CD86 molecules.

Authors:  P S Linsley; S G Nadler; J Bajorath; R Peach; H T Leung; J Rogers; J Bradshaw; M Stebbins; G Leytze; W Brady
Journal:  J Biol Chem       Date:  1995-06-23       Impact factor: 5.157

7.  Oxytocin and vasopressin V1a and V2 receptors form constitutive homo- and heterodimers during biosynthesis.

Authors:  Sonia Terrillon; Thierry Durroux; Bernard Mouillac; Andreas Breit; Mohammed A Ayoub; Magali Taulan; Ralf Jockers; Claude Barberis; Michel Bouvier
Journal:  Mol Endocrinol       Date:  2002-12-23

8.  Single-molecule analysis of fluorescently labeled G-protein-coupled receptors reveals complexes with distinct dynamics and organization.

Authors:  Davide Calebiro; Finn Rieken; Julia Wagner; Titiwat Sungkaworn; Ulrike Zabel; Alfio Borzi; Emanuele Cocucci; Alexander Zürn; Martin J Lohse
Journal:  Proc Natl Acad Sci U S A       Date:  2012-12-24       Impact factor: 11.205

9.  Homo- and hetero-oligomerization of thyrotropin-releasing hormone (TRH) receptor subtypes. Differential regulation of beta-arrestins 1 and 2.

Authors:  Aylin C Hanyaloglu; Ruth M Seeber; Trudy A Kohout; Robert J Lefkowitz; Karin A Eidne
Journal:  J Biol Chem       Date:  2002-10-21       Impact factor: 5.157

10.  Unraveling receptor stoichiometry using bret.

Authors:  James H Felce; Simon J Davis
Journal:  Front Endocrinol (Lausanne)       Date:  2012-07-12       Impact factor: 5.555
View more
  7 in total

1.  Third-Party Capture of Elusive GPCR Dimers.

Authors:  Thomas Huber; Thomas P Sakmar
Journal:  Biophys J       Date:  2018-11-22       Impact factor: 4.033

2.  Conformational biosensors reveal allosteric interactions between heterodimeric AT1 angiotensin and prostaglandin F2α receptors.

Authors:  Rory Sleno; Dominic Devost; Darlaine Pétrin; Alice Zhang; Kyla Bourque; Yuji Shinjo; Junken Aoki; Asuka Inoue; Terence E Hébert
Journal:  J Biol Chem       Date:  2017-06-05       Impact factor: 5.157

3.  Oligomerization of yeast α-factor receptor detected by fluorescent energy transfer between ligands.

Authors:  Sara M Connelly; Rajashri Sridharan; Fred Naider; Mark E Dumont
Journal:  Biophys J       Date:  2021-10-08       Impact factor: 4.033

4.  BRET evidence that β2 adrenergic receptors do not oligomerize in cells.

Authors:  Tien-Hung Lan; Qiuju Liu; Chunman Li; Guangyu Wu; Jan Steyaert; Nevin A Lambert
Journal:  Sci Rep       Date:  2015-05-08       Impact factor: 4.379

5.  Constraints on GPCR Heterodimerization Revealed by the Type-4 Induced-Association BRET Assay.

Authors:  James H Felce; Alasdair MacRae; Simon J Davis
Journal:  Biophys J       Date:  2018-11-22       Impact factor: 4.033

6.  Referenced Single-Molecule Measurements Differentiate between GPCR Oligomerization States.

Authors:  Sarah L Latty; James H Felce; Laura Weimann; Steven F Lee; Simon J Davis; David Klenerman
Journal:  Biophys J       Date:  2015-11-03       Impact factor: 4.033

7.  Receptor Quaternary Organization Explains G Protein-Coupled Receptor Family Structure.

Authors:  James H Felce; Sarah L Latty; Rachel G Knox; Susan R Mattick; Yuan Lui; Steven F Lee; David Klenerman; Simon J Davis
Journal:  Cell Rep       Date:  2017-09-12       Impact factor: 9.423
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.