Akira Hasebe1, Hong-Hua Mu, Barry C Cole. 1. Division of Rheumatology, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, UT, USA; Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
Abstract
PROBLEM: Mycoplasma hominis has been implicated in many inflammatory conditions of the human urogenital tract in particular amniotic infections that lead to fetal and neonatal disease and pre-term labor. The mechanisms responsible are poorly defined. METHOD OF STUDY: Biochemical and immunological methods were used to extract, purify, and characterize an inflammatory component present in M. hominis. RESULTS: We isolated and purified to homogeneity a 40-kDa bioactive lipoprotein from M. hominis that was a potent TLR2-dependent, CD14-independent activator of the human THP-1 macrophage cell line. Homology searches of the N-terminal sequence revealed that 22 of the first 23 residues were identical to those seen for the phase-variable M. hominis p50 adhesin. The truncated P50t lipoprotein importantly retained its adhesive properties for human macrophages. CONCLUSION: The unique adhesin/macrophage activator may play a key role in M. hominis infections by triggering an inflammatory cytokine cascade.
PROBLEM: Mycoplasma hominis has been implicated in many inflammatory conditions of the human urogenital tract in particular amniotic infections that lead to fetal and neonatal disease and pre-term labor. The mechanisms responsible are poorly defined. METHOD OF STUDY: Biochemical and immunological methods were used to extract, purify, and characterize an inflammatory component present in M. hominis. RESULTS: We isolated and purified to homogeneity a 40-kDa bioactive lipoprotein from M. hominis that was a potent TLR2-dependent, CD14-independent activator of the humanTHP-1 macrophage cell line. Homology searches of the N-terminal sequence revealed that 22 of the first 23 residues were identical to those seen for the phase-variable M. hominis p50 adhesin. The truncated P50t lipoprotein importantly retained its adhesive properties for human macrophages. CONCLUSION: The unique adhesin/macrophage activator may play a key role in M. hominis infections by triggering an inflammatory cytokine cascade.
Authors: J Goret; L Béven; B Faustin; C Contin-Bordes; C Le Roy; S Claverol; H Renaudin; C Bébéar; S Pereyre Journal: J Bacteriol Date: 2017-07-11 Impact factor: 3.490
Authors: Gleb Yu Fisunov; Olga V Pobeguts; Valentina G Ladygina; Alexandr I Zubov; Mariya A Galyamina; Sergey I Kovalchuk; Rustam K Ziganshin; Daria V Evsyutina; Daria S Matyushkina; Ivan O Butenko; Olga N Bukato; Vladimir A Veselovsky; Tatiana A Semashko; Ksenia M Klimina; Galina A Levina; Olga I Barhatova; Irina V Rakovskaya Journal: J Med Microbiol Date: 2022-01 Impact factor: 2.472