Ali Osman Yıldırım1, Mehmet Eryılmaz2, Umit Kaldırım2, Yusuf Emrah Eyi3, Salim Kemal Tuncer2, Murat Eroğlu4, Murat Durusu2, Turgut Topal5, Bülent Kurt6, Serkan Dilmen7, Serkan Bilgiç8, Muhittin Serdar9. 1. Department of Emergency Medicine, GATA, Haydarpasa Military Hosital, İstanbul, Turkey. draliosmanyildirim@gmail.com. 2. Department of Emergency Medicine, GATA School of Medicine, Ankara, Turkey. 3. Department of Emergency Medicine, Hakkari Military Hospital, Hakkari, Turkey. 4. Department of Emergency Medicine, GATA, Haydarpasa Military Hosital, İstanbul, Turkey. 5. Department of Physiology, GATA School of Medicine, Ankara, Turkey. 6. Department of Pathology, GATA School of Medicine, Ankara, Turkey. 7. Department of Emergency Medicine, Elazığ Military Hospital, Elazığ, Turkey. 8. Department of Orthopaedics and Traumatology, GATA, Haydarpaşa Military Hosital, İstanbul, Turkey. 9. Department of Biochemistry, GATA School of Medicine, Ankara, Turkey.
Abstract
BACKGROUND: Soft tissue trauma is a type of acute traumatic ischemia. We investigated in this study whether the edema, inflammation and ischemia caused by the trauma could be affected positively by hyperbaric oxygen (HBO) and ozone therapy. METHODS: Soft tissue trauma was generated in a total of 63 adult male Sprague-Dawley rats. Subsequently, rats were divided into three groups. The first group was treated with ozone, the second group with HBO, and the third group served as controls. Tissue and blood samples were taken at the end of the procedures. Tissue lipid peroxidation (LPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), inducible nitric oxide synthase (iNOS), heme oxygenase (HO)-1, and hypoxia-inducible factor (HIF)-1 levels were detected. Hematoxylin-eosin staining was used to determine the inflammation and edema histopathologically. RESULTS: We also detected HIF-1 activity, which decreases when the oxygen concentration increases, HO-1 activity, which has anti-inflammatory effects, and iNOS activity, which releases in any type of acute case. We determined a statistically significant reduction in iNOS and LPO levels in both the HBO and Ozone groups. A significant decrease in inflammation was detected in both the Ozone and HBO groups compared with the Control group, and a significant decrease in edema was detected in all three groups. CONCLUSION: We think that HBO and Ozone therapy have beneficial effects on biochemical and histopathological findings. Related clinical trials will be helpful in clarifying the effects.
BACKGROUND: Soft tissue trauma is a type of acute traumatic ischemia. We investigated in this study whether the edema, inflammation and ischemia caused by the trauma could be affected positively by hyperbaric oxygen (HBO) and ozone therapy. METHODS: Soft tissue trauma was generated in a total of 63 adult male Sprague-Dawley rats. Subsequently, rats were divided into three groups. The first group was treated with ozone, the second group with HBO, and the third group served as controls. Tissue and blood samples were taken at the end of the procedures. Tissue lipid peroxidation (LPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), inducible nitric oxide synthase (iNOS), heme oxygenase (HO)-1, and hypoxia-inducible factor (HIF)-1 levels were detected. Hematoxylin-eosin staining was used to determine the inflammation and edema histopathologically. RESULTS: We also detected HIF-1 activity, which decreases when the oxygen concentration increases, HO-1 activity, which has anti-inflammatory effects, and iNOS activity, which releases in any type of acute case. We determined a statistically significant reduction in iNOS and LPO levels in both the HBO and Ozone groups. A significant decrease in inflammation was detected in both the Ozone and HBO groups compared with the Control group, and a significant decrease in edema was detected in all three groups. CONCLUSION: We think that HBO and Ozone therapy have beneficial effects on biochemical and histopathological findings. Related clinical trials will be helpful in clarifying the effects.
Authors: Suzan Bayer; Hakki Oguz Kazancioglu; Ahmet Hüseyin Acar; Nihat Demirtas; Nur Ozten Kandas Journal: Lasers Med Sci Date: 2017-02-11 Impact factor: 3.161
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