Literature DB >> 2493397

Identification of both Gi2 and a novel, immunologically distinct, form of Go in rat myometrial membranes.

G Milligan1, Z Tanfin, O Goureau, C Unson, S Harbon.   

Abstract

Immunoblotting of rat myometrial membranes with an antiserum (SG1) which recognizes the alpha-subunits of both Gi1 and Gi2 indicated the presence of detectable levels of an apparently single form of some 40 kDa. A second antiserum (LE2) specific for Gi2 also recognized this protein, confirming its identity. Immunoblotting of the myometrial membranes with a series of antipeptide (OC1, IM1, ON1) and polyclonal (RV3) antisera, all of which recognize rat brain Go, produced a more complex pattern. Antisera OC1 and ON1 recognized a single polypeptide which essentially comigrated with rat brain Go. In contrast, antisera RV3 and IM1 did not recognize the myometrial protein. These data provide evidence for the presence of Gi2 and of a novel G-protein, related immunologically to Go, in rat myometrial membranes.

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Year:  1989        PMID： 2493397     DOI： 10.1016/0014-5793(89)80574-5

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  10 in total

1.  Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go.

Authors:  M Strathmann; T M Wilkie; M I Simon
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

Review 2.  Molecular mechanisms of go signaling.

Authors:  Meisheng Jiang; Neil S Bajpayee
Journal:  Neurosignals       Date:  2009-02-12

3.  Guanine-nucleotide-binding proteins expressed in rat white adipose tissue. Identification of both mRNAs and proteins corresponding to Gi1, Gi2 and Gi3.

Authors:  F M Mitchell; S L Griffiths; E D Saggerson; M D Houslay; J T Knowler; G Milligan
Journal:  Biochem J       Date:  1989-09-01       Impact factor: 3.857

4.  Cholecystokinin activates Gi1-, Gi2-, Gi3- and several Gs-proteins in rat pancreatic acinar cells.

Authors:  S Schnefel; A Pröfrock; K D Hinsch; I Schulz
Journal:  Biochem J       Date:  1990-07-15       Impact factor: 3.857

5.  Alternative splicing of the guanine nucleotide-binding regulatory protein Go alpha generates four distinct mRNAs.

Authors:  J J Murtagh; J Moss; M Vaughan
Journal:  Nucleic Acids Res       Date:  1994-03-11       Impact factor: 16.971

6.  Characterization of heterotrimeric G-proteins in adult Acanthocheilonema viteae.

Authors:  K R Grant; M M Harnett; G Milligan; W Harnett
Journal:  Biochem J       Date:  1996-12-01       Impact factor: 3.857

7.  Prostaglandins and muscarinic agonists induce cyclic AMP attenuation by two distinct mechanisms in the pregnant-rat myometrium. Interaction between cyclic AMP and Ca2+ signals.

Authors:  O Goureau; Z Tanfin; S Harbon
Journal:  Biochem J       Date:  1990-11-01       Impact factor: 3.857

8.  Cellular distribution and biochemical characterization of G proteins in skeletal muscle: comparative location with voltage-dependent calcium channels.

Authors:  M Toutant; J Gabrion; S Vandaele; S Peraldi-Roux; J Barhanin; J Bockaert; B Rouot
Journal:  EMBO J       Date:  1990-02       Impact factor: 11.598

9.  Different forms of Go alpha mRNA arise by alternative splicing of transcripts from a single gene on human chromosome 16.

Authors:  J J Murtagh; R Eddy; T B Shows; J Moss; M Vaughan
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272

10.  Structure of the human gene and two rat cDNAs encoding the alpha chain of GTP-binding regulatory protein Go: two different mRNAs are generated by alternative splicing.

Authors:  T Tsukamoto; R Toyama; H Itoh; T Kozasa; M Matsuoka; Y Kaziro
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205
  10 in total

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