Olivier Bausset1, Jeremy Magalon1, Laurent Giraudo1, Marie-Laure Louis2, Nicolas Serratrice3, Corrine Frere4, Guy Magalon3, Françoise Dignat-George5, Florence Sabatier5. 1. Culture and Therapy Unit, Assistance Publique des Hôpitaux de Marseille, France. 2. Orthopaedic Surgery Unit, CHU La Conception, Assistance Publique des Hôpitaux de Marseille, France. 3. Department of Plastic and Reconstructive Surgery, CHU La Conception, Assistance Publique des Hôpitaux de Marseille, France. 4. Faculty of Pharmacy, Inserm UMR-S1076, Marseille, France. 5. Culture and Therapy Unit, Assistance Publique des Hôpitaux de Marseille, France ; Faculty of Pharmacy, Inserm UMR-S1076, Marseille, France.
Abstract
UNLABELLED: The platelet-rich plasma (PRP) is an autologous biotherapy commonly used for its healing properties. Once activated, platelets released a real "cocktail" of growth factor and cytokines implied in numerous regenerative processes. However the impact of medical practices associated to PRP therapeutic use on platelets functionality remains poorly known. OBJECTIVES: we evaluated the in vitro effects of two commonly used local anesthetics (Xylocaine(*) and Naropin(*)) on PRP functionality. We also investigated the quantity and quality of PRP that passed through the smallest gauge needle commercialized. MATERIALS AND METHODS: PRP from 9 healthy volunteers were prepared using our previously described home made purification protocol. Platelet aggregation capacity was evaluated by aggregometry assays and the growth factor release was determined by ELISA after platelet activation. We also evaluated the platelet activation status, reactivity and stability of platelets by flow cytometry using the P-selectin expression marker. RESULTS: the association of local anaesthetics with PRP injections resulted in a significant decrease of platelets functionality, assessed by their capacity of aggregating. Local anaesthetics did not interfere with the growth factor release. The different needle sizes and calibres tested for PRP injections did not influence the platelet functionality. CONCLUSIONS: the use of local anaesthetics to prevent pain during PRP injections could compromise the therapeutic potential of PRP. These results suggest using carefully local anaesthetics or limiting their use as often is possible. To minimize injection pain, we recommend using 30 G needles. These data will lead to clinical recommendations for painless and controlled PRP injections.
UNLABELLED: The platelet-rich plasma (PRP) is an autologous biotherapy commonly used for its healing properties. Once activated, platelets released a real "cocktail" of growth factor and cytokines implied in numerous regenerative processes. However the impact of medical practices associated to PRP therapeutic use on platelets functionality remains poorly known. OBJECTIVES: we evaluated the in vitro effects of two commonly used local anesthetics (Xylocaine(*) and Naropin(*)) on PRP functionality. We also investigated the quantity and quality of PRP that passed through the smallest gauge needle commercialized. MATERIALS AND METHODS: PRP from 9 healthy volunteers were prepared using our previously described home made purification protocol. Platelet aggregation capacity was evaluated by aggregometry assays and the growth factor release was determined by ELISA after platelet activation. We also evaluated the platelet activation status, reactivity and stability of platelets by flow cytometry using the P-selectin expression marker. RESULTS: the association of local anaesthetics with PRP injections resulted in a significant decrease of platelets functionality, assessed by their capacity of aggregating. Local anaesthetics did not interfere with the growth factor release. The different needle sizes and calibres tested for PRP injections did not influence the platelet functionality. CONCLUSIONS: the use of local anaesthetics to prevent pain during PRP injections could compromise the therapeutic potential of PRP. These results suggest using carefully local anaesthetics or limiting their use as often is possible. To minimize injection pain, we recommend using 30 G needles. These data will lead to clinical recommendations for painless and controlled PRP injections.
Entities:
Keywords:
PRP; Platelet concentrate local anaesthetics; growth factors; platelet-rich plasma; regenerative medicine; sport medicine; tendinopathy
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