Min-Soo Kim1, Joo-Young Kwon2, Jung-Seok Lee3, Je Seon Song4, Seong-Ho Choi5, Ui-Won Jung6. 1. Clinical Research Assistant Professor, Department of Periodontology, College of Dentistry, Yonsei University, Seoul, South Korea. 2. Graduate Student, Department of Periodontology, College of Dentistry, Yonsei University, Seoul, South Korea. 3. Clinical Assistant Professor, Department of Periodontology, College of Dentistry, Yonsei University, Seoul, South Korea. 4. Associate Professor, Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, South Korea. 5. Professor, Department of Periodontology, College of Dentistry, Yonsei University, Seoul, South Korea. 6. Associate Professor, Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, South Korea. Electronic address: drjew@yuhs.ac.
Abstract
PURPOSE: The objectives of this study were to confirm the osteogenic potential of the Schneiderian membrane and to elucidate the early healing pattern of low-dose recombinant human bone morphogenetic protein-2 (rhBMP-2)-coated biphasic calcium phosphate (BCP). MATERIALS AND METHODS: The osteogenic potential of the Schneiderian membrane and enhancement by rhBMP-2 were evaluated by in vitro analysis. RhBMP-2-coated BCP (experimental group) and BCP soaked with saline (control group) were applied to the maxillary sinus in rabbits. After 2 weeks, micro-computed tomographic and histometric analyses were performed. RESULTS: Enhanced osteogenic potential was found when cells from the Schneiderian membrane were treated with rhBMP-2. Micro-computed tomographic analysis showed that the total augmented volume was significantly larger in the experimental group. Different healing patterns were observed in 3 regions, although the area of new bone did not differ significantly. Although more newly formed bone appeared, particularly along the Schneiderian membrane in the experimental group, the difference was not statistically significant. CONCLUSIONS: RhBMP-2 enhanced the osteogenic potential of the Schneiderian membrane in vitro. However, low-dose rhBMP-2-coated BCP failed to exert a statistically significant effect in vivo, although it appeared to be effective in sinus augmentation specifically for the volumetric increase in the early phase.
PURPOSE: The objectives of this study were to confirm the osteogenic potential of the Schneiderian membrane and to elucidate the early healing pattern of low-dose recombinant humanbone morphogenetic protein-2 (rhBMP-2)-coated biphasic calcium phosphate (BCP). MATERIALS AND METHODS: The osteogenic potential of the Schneiderian membrane and enhancement by rhBMP-2 were evaluated by in vitro analysis. RhBMP-2-coated BCP (experimental group) and BCP soaked with saline (control group) were applied to the maxillary sinus in rabbits. After 2 weeks, micro-computed tomographic and histometric analyses were performed. RESULTS: Enhanced osteogenic potential was found when cells from the Schneiderian membrane were treated with rhBMP-2. Micro-computed tomographic analysis showed that the total augmented volume was significantly larger in the experimental group. Different healing patterns were observed in 3 regions, although the area of new bone did not differ significantly. Although more newly formed bone appeared, particularly along the Schneiderian membrane in the experimental group, the difference was not statistically significant. CONCLUSIONS: RhBMP-2 enhanced the osteogenic potential of the Schneiderian membrane in vitro. However, low-dose rhBMP-2-coated BCP failed to exert a statistically significant effect in vivo, although it appeared to be effective in sinus augmentation specifically for the volumetric increase in the early phase.