Literature DB >> 24929037

A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches.

Eoin Gunnigle1, Jean-Baptiste Ramond1, Aline Frossard1, Mary Seeley2, Don Cowan3.   

Abstract

A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.
Copyright © 2014 Elsevier B.V. All rights reserved.

Keywords:  Co-extraction; Metaproteomics; Nucleic acids; Protein; T-RFLP

Mesh：

Substances：

Year:  2014        PMID： 24929037     DOI： 10.1016/j.mimet.2014.06.004

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  8 in total

Review 1.  Microbiomics of Namib Desert habitats.

Authors:  D A Cowan; D W Hopkins; B E Jones; G Maggs-Kölling; R Majewska; J-B Ramond
Journal:  Extremophiles       Date:  2019-08-02       Impact factor: 2.395

2.  Namib Desert edaphic bacterial, fungal and archaeal communities assemble through deterministic processes but are influenced by different abiotic parameters.

Authors:  Riegardt M Johnson; Jean-Baptiste Ramond; Eoin Gunnigle; Mary Seely; Don A Cowan
Journal:  Extremophiles       Date:  2017-01-05       Impact factor: 2.395

Review 3.  Microbial Biogeochemical Cycling of Nitrogen in Arid Ecosystems.

Authors:  Jean-Baptiste Ramond; Karen Jordaan; Beatriz Díez; Sandra M Heinzelmann; Don A Cowan
Journal:  Microbiol Mol Biol Rev       Date:  2022-04-07       Impact factor: 13.044

4.  Niche-partitioning of edaphic microbial communities in the Namib Desert gravel plain Fairy Circles.

Authors:  Jean-Baptiste Ramond; Annelize Pienaar; Alacia Armstrong; Mary Seely; Don A Cowan
Journal:  PLoS One       Date:  2014-10-03       Impact factor: 3.240

5.  Diel-scale temporal dynamics recorded for bacterial groups in Namib Desert soil.

Authors:  Eoin Gunnigle; Aline Frossard; Jean-Baptiste Ramond; Leandro Guerrero; Mary Seely; Don A Cowan
Journal:  Sci Rep       Date:  2017-01-10       Impact factor: 4.379

Review 6.  Soil Metaproteomics for the Study of the Relationships Between Microorganisms and Plants: A Review of Extraction Protocols and Ecological Insights.

Authors:  Maria Tartaglia; Felipe Bastida; Rosaria Sciarrillo; Carmine Guarino
Journal:  Int J Mol Sci       Date:  2020-11-11       Impact factor: 5.923

7.  The guanidine thiocyanate-high EDTA method for total microbial RNA extraction from severely heavy metal-contaminated soils.

Authors:  Yaxin Pei; Tursunay Mamtimin; Jing Ji; Aman Khan; Apurva Kakade; Tuoyu Zhou; Zhengsheng Yu; Hajira Zain; Wenzhi Yang; Zhenmin Ling; Wenya Zhang; Yingmei Zhang; Xiangkai Li
Journal:  Microb Biotechnol       Date:  2020-06-23       Impact factor: 5.813

Review 8.  Soil and leaf litter metaproteomics-a brief guideline from sampling to understanding.

Authors:  Katharina M Keiblinger; Stephan Fuchs; Sophie Zechmeister-Boltenstern; Katharina Riedel
Journal:  FEMS Microbiol Ecol       Date:  2016-08-21       Impact factor: 4.194
  8 in total

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