Literature DB >> 24928421

Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2.

E Pileri1, M Cortey2, F Rodríguez3, M Sibila3, L Fraile4, J Segalés5.   

Abstract

The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n = 1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results was excellent (r(2) ≥ 0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%), followed by E3 (90.1%) and E1 (85.0%); the specificity was 100% for all tests. All three commercial ELISAs had predictive values similar to those of IPMA and could be used to monitor antibody responses against PCV2 infection and/or vaccination.
Copyright © 2014 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  ELISA; Immunoperoxidase monolayer assay; Porcine circovirus type 2; Serology

Mesh：

Substances：

Year:  2014        PMID： 24928421     DOI： 10.1016/j.tvjl.2014.05.025

Source DB:  PubMed          Journal:  Vet J        ISSN： 1090-0233            Impact factor:   2.688


  4 in total

1.  An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies.

Authors:  Andy Haegeman; Ilse De Leeuw; Laurent Mostin; Willem Van Campe; Laetitia Aerts; Maria Vastag; Kris De Clercq
Journal:  J Virol Methods       Date:  2019-12-16       Impact factor: 2.014

2.  Combined Antibody Tagged HRP Gold Nanoparticle Probe for Effective PCV2 Screening in Pig Farms.

Authors:  Shouping Zhang; Lei Wang; Lirong Wang; Nan Yu; Yongjun Dong; Jianhe Hu
Journal:  Int J Nanomedicine       Date:  2022-07-29

3.  Descriptive analyses of maternally-derived antibody levels against porcine circovirus 2 (PCV-2) in 3- and 21-day-old piglets from farms of four European countries using different vaccination protocols in sows.

Authors:  M Sibila; A Llorens; E Huerta; C Fablet; M Faderl; L Ferrari; N Rose; A Palzer; P Martelli; M C Venegas-Vargas; D Fredrickson; L Taylor; M Balasch; M Bandrick; J Segalés
Journal:  Porcine Health Manag       Date:  2022-10-03

4.  Porcine circovirus 2 (PCV2) population study in experimentally infected pigs developing PCV2-systemic disease or a subclinical infection.

Authors:  Florencia Correa-Fiz; Giovanni Franzo; Anna Llorens; Eva Huerta; Marina Sibila; Tuija Kekarainen; Joaquim Segalés
Journal:  Sci Rep       Date:  2020-10-20       Impact factor: 4.379
  4 in total

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