| Literature DB >> 24927835 |
Sho Maekawa1, Ayako Suzuki, Sumio Sugano, Yutaka Suzuki.
Abstract
The introduction of RNA sequencing (RNA-seq), as a direct result of rapid progression of next-generation sequencing technologies, has revolutionized the world of transcriptomics. It enables quantification of the complete set of RNA with all its isoforms in a given cell, in far greater accuracy than before. Here, we provide a step-by-step guide to the sample preparation in RNA-seq and an overview of the bioinformatic analysis that is required following the cDNA sequencing.Mesh：
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Year: 2014 PMID： 24927835 DOI： 10.1007/978-1-4939-0805-9_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745