Chromatin immunoprecipitation protocol for mammalian cells.

The genomic approach (ChIP-seq) we introduce here is now a widely used powerful tool to explore protein-DNA interaction at genome-wide level in high resolution. This technology opens up the way to understand how local event mediated by protein-protein or protein-DNA interactions lead to the dynamic changes of overall chromosome structure and how variety of proteins make a regulatory network for the faithful execution of various chromosomal functions (i.e., transcription, replication, recombination, repair, and partition).