Literature DB >> 2492583

Detection of antibodies to extractable nuclear antigens using calf thymus and rabbit thymus. A comparative study of 1000 consecutive anti-nuclear antibody positive patients.

R J Collins1, J C Neil, L N Druery, R J Wilson.   

Abstract

A comparison between calf thymus extract and rabbit thymus extract to determine their suitability for clinical laboratories in detecting antibodies to extractable nuclear antigens (ENA) was undertaken. Calf thymus extract (CTE) detected 90% more ENA positive patients and 155% more lines than rabbit thymus extract (RTE). In contrast to RTE, CTE resolved Sm and RNP into two distinct lines, had superior stability at -80 degrees C and produced greater clarity of definition of precipitin lines.

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Year:  1989        PMID: 2492583     DOI: 10.1016/0022-1759(89)90312-8

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  3 in total

Review 1.  Antinuclear antibody determination methods.

Authors:  D G Williams; P J Charles; M Field; S M Chua; R N Maini
Journal:  Clin Rheumatol       Date:  1990-03       Impact factor: 2.980

2.  Autoantibodies in patients with juvenile chronic arthritis and their immediate family relatives.

Authors:  T R Southwood; P J Roberts-Thomson; M J Ahern; K Shepherd; R McEvoy; J B Ziegler; J Edmonds
Journal:  Ann Rheum Dis       Date:  1990-12       Impact factor: 19.103

3.  Specific testing for "isolated" anti-52 kDa SSA/Ro antibodies during standard anti-extractable nuclear antigen testing is of limited clinical value.

Authors:  Daman M Langguth; Samantha Morris; Lynette Clifford; Robert J Wilson; John Neil; Patrick G Hogan; Richard C W Wong
Journal:  J Clin Pathol       Date:  2007-06       Impact factor: 3.411

  3 in total

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