Effects of interleukin-10 gene deficiency on hepatic biochemical metabolism in mice.

The aim of this study was to investigate the effect of interleukin-10 (IL-10) gene deficiency on mouse liver function. The experimental mice were divided into wild-type and IL-10 knockout groups. Serological biomarkers for liver functions were detected by the automatic biochemical analyzer AU5400. The pathological changes were assessed by the light microscope. The levels of inducible nitric oxide synthase (iNOS) and interleukin-1β (IL-1β) in liver tissues were determined by quantitative real-time PCR and enzyme-linked immunosorbent assay. Compared with the wild type, the serum levels of albumin (ALB), total protein, total bilirubin and direct bilirubin in IL-10-deficient mice were significantly decreased (P < 0.05). No obvious pathological changes including liver necrosis and inflammatory cell infiltration were found. The expression of iNOS and IL-1β genes, the serum levels of iNOS and IL-1β were significantly higher in IL-10-deficient mice than in wild-type mice (P < 0.05). The absence of IL-10 gene can significantly decrease serum ALB and bilirubin. The effect may be related to the upregulation of iNOS and IL-1β.