| Literature DB >> 2492439 |
K Takegawa1, N Kawasaki, S Iwahara, K Yamamoto, T Tochikura, B Mikami, Y Morita.
Abstract
The primary structure of the N-linked sugar chain of Rhizopus niveus glucoamylase (major component) was investigated. The carbohydrate moiety was released from the polypeptide backbone by Flavobacterium sp. endo-beta-N-acetylglucosaminidase digestion. Studies using the method of exoglycosidase digestion of the fluorescent pyridylamino derivative, gel-permeation chromatography on Bio-Gel P-4 and 400-MHz 1H-NMR spectroscopy revealed that the most abundant structure is (Man)8-GlcNac-ol.Entities:
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Year: 1989 PMID： 2492439 DOI： 10.1016/s0304-4165(89)80018-2
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002