PURPOSE: To test the ability of different magnetic resonance imaging (MRI) modalities to discriminate the time course of damage and regeneration in a model of acute, toxin-induced muscle damage. MATERIALS AND METHODS: We analyzed the time course of tissue and cellular changes in mouse lower limb musculature following localized injection of myotoxin by T2 , magnetization transfer (MT), and diffusion-weighted MRI. We also used T1 -weighted imaging to measure leg muscle volume. In addition, postmortem histological analysis of toxin-injected muscles was compared to uninjected controls. RESULTS: The damages detected by the MRI modalities are transient and recover within 3 weeks. Muscle water diffusivity and edema measured by leg volume increased within the first hours after injection of the toxin. The rate constant for volume increase was 0.65 ± 0.11 hr(-1) , larger than the increase in T2 (0.045 ± 0.013 hr(-1) ) and change in MT ratio (0.028 ± 0.021 hr(-1) ). During repair phase, the rate constants were much smaller: 0.022 ± 0.004 hr(-1) , 0.013 ± 0.0019 hr(-1) and 0.0042 ± 0.0016 hr(-1) for volume, T2 , and MT ratio, respectively. Histological analyses confirmed the underlying cellular changes that matched the progression of MR images. CONCLUSION: The kinetics of change in the MRI measurements during the progression of damage and repair shows MRI modalities can be used to distinguish these processes.
PURPOSE: To test the ability of different magnetic resonance imaging (MRI) modalities to discriminate the time course of damage and regeneration in a model of acute, toxin-induced muscle damage. MATERIALS AND METHODS: We analyzed the time course of tissue and cellular changes in mouse lower limb musculature following localized injection of myotoxin by T2 , magnetization transfer (MT), and diffusion-weighted MRI. We also used T1 -weighted imaging to measure leg muscle volume. In addition, postmortem histological analysis of toxin-injected muscles was compared to uninjected controls. RESULTS: The damages detected by the MRI modalities are transient and recover within 3 weeks. Muscle water diffusivity and edema measured by leg volume increased within the first hours after injection of the toxin. The rate constant for volume increase was 0.65 ± 0.11 hr(-1) , larger than the increase in T2 (0.045 ± 0.013 hr(-1) ) and change in MT ratio (0.028 ± 0.021 hr(-1) ). During repair phase, the rate constants were much smaller: 0.022 ± 0.004 hr(-1) , 0.013 ± 0.0019 hr(-1) and 0.0042 ± 0.0016 hr(-1) for volume, T2 , and MT ratio, respectively. Histological analyses confirmed the underlying cellular changes that matched the progression of MR images. CONCLUSION: The kinetics of change in the MRI measurements during the progression of damage and repair shows MRI modalities can be used to distinguish these processes.
Authors: Q Y Gong; J Phoenix; G J Kemp; M García-Fiñana; S P Frostick; D A Brodie; R H Edwards; G H Whitehouse; N Roberts Journal: J Magn Reson Imaging Date: 2000-09 Impact factor: 4.813
Authors: Tobias Winkler; Philipp von Roth; Georg Matziolis; Maria R Schumann; Sebastian Hahn; Patrick Strube; Gisela Stoltenburg-Didinger; Carsten Perka; Georg N Duda; Stephan V Tohtz Journal: Acta Orthop Date: 2010-12-13 Impact factor: 3.717
Authors: Gustav J Strijkers; Ericky C A Araujo; Noura Azzabou; David Bendahan; Andrew Blamire; Jedrek Burakiewicz; Pierre G Carlier; Bruce Damon; Xeni Deligianni; Martijn Froeling; Arend Heerschap; Kieren G Hollingsworth; Melissa T Hooijmans; Dimitrios C Karampinos; George Loudos; Guillaume Madelin; Benjamin Marty; Armin M Nagel; Aart J Nederveen; Jules L Nelissen; Francesco Santini; Olivier Scheidegger; Fritz Schick; Christopher Sinclair; Ralph Sinkus; Paulo L de Sousa; Volker Straub; Glenn Walter; Hermien E Kan Journal: J Neuromuscul Dis Date: 2019
Authors: Germana Zaccagnini; Anna Palmisano; Tamara Canu; Biagina Maimone; Francesco M Lo Russo; Federico Ambrogi; Carlo Gaetano; Francesco De Cobelli; Alessandro Del Maschio; Antonio Esposito; Fabio Martelli Journal: PLoS One Date: 2015-11-10 Impact factor: 3.240
Authors: Joshua S Park; Ravneet Vohra; Thomas Klussmann; Niclas E Bengtsson; Jeffrey S Chamberlain; Donghoon Lee Journal: PLoS One Date: 2018-10-26 Impact factor: 3.752