Literature DB >> 2492301

Evaluation of a method for rapid detection of penicillinase-producing Neisseria gonorrhoeae in urethral exudates.

V M Herve1, A J Georges, M Massanga, P M Martin.   

Abstract

A substrate, pyridine-2-azo-p-dimethylaniline cephalosporin (PADAC; Diagnostic Pasteur, Marnes-La-Coquette, France), for detection of penicillinase-producing Neisseria gonorrhoeae (PPNG) on isolated colonies grown on agar was compared with the nitrocefin reference test (Cefinase; Biomerieux, Marcy l'Etoile, France). The PADAC test was also used to detect PPNG directly on urethral exudates. All 38 PPNG-containing and 29 of 30 PPNG-negative urethral pus isolates gave results in agreement with those obtained on cultured isolates. We conclude that direct detection of PPNG in urethral exudates from males is simple, rapid, reliable, and sensitive.

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Year:  1989        PMID： 2492301      PMCID： PMC267275          DOI： 10.1128/jcm.27.1.227-228.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Enhancement of fluorescence development of end products by use of a fluorescence developer solution in a rapid and sensitive fluorescent spot test for specific detection of microbial beta-lactamases.

Authors:  K C Chen; K K Holmes
Journal:  J Clin Microbiol       Date:  1986-03       Impact factor: 5.948

2.  Beta-lactamase-producing, penicillin-resistant gonococcus.

Authors:  I Phillips
Journal:  Lancet       Date:  1976-09-25       Impact factor: 79.321

3.  Rapid, inexpensive method for specific detection of microbial beta-lactamases by detection of fluorescent end products.

Authors:  K C Chen; J S Knapp; K K Holmes
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

4.  Rapid identification of penicillinase-producing Neisseria gonorrhoeae by detection of beta-lactamase in urethral exudates.

Authors:  D N Taylor; K C Chen; K Panikabutra; C Wongba; A Chitwarakern; P Echeverria; K K Holmes
Journal:  Lancet       Date:  1985-09-21       Impact factor: 79.321

5.  In vitro evaluation of pyridine-2-azo-p-dimethylaniline cephalosporin, a new diagnostic chromogenic reagent, and comparison with nitrocefin, cephacetrile, and other beta-lactam compounds.

Authors:  R N Jones; H W Wilson; W J Novick
Journal:  J Clin Microbiol       Date:  1982-04       Impact factor: 5.948
  5 in total

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