Literature DB >> 2492299

Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp.

C M Smitka1, S G Jackson.   

Abstract

A 30-min fluorogenic test was developed for differentiation of members of the Candida parapsilosis group from other Candida species commonly encountered in clinical material. The fluorogenic substrate, 4-methylumbelliferyl-beta-D-glucoside, was utilized to assay beta-glucosidase activity. A total of 50 C. parapsilosis isolates and 135 isolates of four other Candida species were tested. Assay sensitivity and specificity were 100 and 99.3%, respectively. The procedure was adapted for use with a spectrofluorometer.

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Year:  1989        PMID: 2492299      PMCID: PMC267266          DOI: 10.1128/jcm.27.1.203-206.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  8 in total

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Journal:  J Clin Pathol       Date:  1964-07       Impact factor: 3.411

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Authors:  D G Bobey; G M Ederer
Journal:  J Clin Microbiol       Date:  1981-02       Impact factor: 5.948

4.  Artificial substrates in the assay of acid glycosidases.

Authors:  B Hultbery; P A Ockerman
Journal:  Clin Chim Acta       Date:  1972-06       Impact factor: 3.786

5.  Umbelliferyl-labeled galactosaminide as an aid in identification of Candida albicans.

Authors:  J L Perry; G R Miller
Journal:  J Clin Microbiol       Date:  1987-12       Impact factor: 5.948

6.  beta-Glucosidase in Candida albicans and its application in yeast identification.

Authors:  I Polacheck; M Melamed; H Bercovier; I F Salkin
Journal:  J Clin Microbiol       Date:  1987-05       Impact factor: 5.948

7.  Candida sepsis. Implications of polymicrobial blood-borne infection.

Authors:  D L Dyess; R N Garrison; D E Fry
Journal:  Arch Surg       Date:  1985-03

8.  Candidiasis. A diagnostic and therapeutic challenge.

Authors:  C A Kauffman; P G Jones
Journal:  Postgrad Med       Date:  1986-07       Impact factor: 3.840

  8 in total
  7 in total

Review 1.  Fluorogenic and chromogenic substrates used in bacterial diagnostics.

Authors:  M Manafi; W Kneifel; S Bascomb
Journal:  Microbiol Rev       Date:  1991-09

2.  Candida albicans colony identification in 5 minutes in a general microbiology laboratory.

Authors:  S F Dealler
Journal:  J Clin Microbiol       Date:  1991-05       Impact factor: 5.948

3.  Utility of Albicans ID plate for rapid identification of Candida albicans in clinical samples. Rapid identification of Candida albicans.

Authors:  I Contreras; R San-Millán; A Agustín-Barrasa; J Pontón; G Quindós
Journal:  Mycopathologia       Date:  1996       Impact factor: 2.574

4.  Comparison of the MUREX C. albicans, Albicans-Sure, and BactiCard Candida test kits with the germ tube test for presumptive identification of Candida albicans.

Authors:  A E Crist; T J Dietz; K Kampschroer
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

5.  Rapid, colorimetric identification of Candida albicans.

Authors:  J L Perry; G R Miller; D L Carr
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

6.  New aniline blue dye medium for rapid identification and isolation of Candida albicans.

Authors:  M C Goldschmidt; D Y Fung; R Grant; J White; T Brown
Journal:  J Clin Microbiol       Date:  1991-06       Impact factor: 5.948

7.  Rapid identification of Candida albicans by using Albicans ID and fluoroplate agar plates.

Authors:  P Rousselle; A M Freydiere; P J Couillerot; H de Montclos; Y Gille
Journal:  J Clin Microbiol       Date:  1994-12       Impact factor: 5.948

  7 in total

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