Cintia Rodrigues Marques1, Thiago Magalhães Da Silva2, Dulcineia Martins De Albuquerque3, Meiryane Santos Chaves1, Marcilio Ferreira Marques Filho1, Jamille Silva Oliveira1, Giuliano Di Pietro4, Sandra Mara Bispo Sousa5, Aguinaldo Luiz Simões6, Fabrício Rios-Santos7. 1. Laboratory of Pharmacogenomics and Genetic Epidemiology (LAFEM), Department of Health, State University of Santa Cruz (UESC), Ilhéus, Brazil. 2. Laboratory of Human Genetics, Department of Biology, State University of Southwest Bahia (UESB), Jequié, Brazil thiago@uesb.edu.br. 3. Hematology and Hemotherapy Center, University of Campinas (UNICAMP), Campinas, Brazil. 4. Laboratory of Genetics, Federal University of Sergipe (UFS), Lagarto, Brazil. 5. Department of Natural Sciences, State University of Southwest Bahia (UESB), Vitória da Conquista, Brazil. 6. Faculty of Medicine of Ribeirão Preto, University of São Paulo, Department of Genetics, Ribeirão Preto, Brazil. 7. Laboratory of Physiology, Faculty of Medicine, Federal University of Mato Grosso (UFMT), Cuiabá, Brazil.
Abstract
AIM: To evaluate associations between polymorphisms of the N-acetyltransferase 2 (NAT2), human 8-oxoguanine glycosylase 1 (hOGG1) and X-ray repair cross-complementing protein 1 (XRCC1) genes and risk of upper aerodigestive tract (UADT) cancer. PATIENTS AND METHODS: A case-control study involving 117 cases and 224 controls was undertaken. The NAT2 gene polymorphisms were genotyped by automated sequencing and XRCC1 Arg399Gln and hOGG1 Ser326Cys polymorphisms were determined by Polymerase Chain Reaction followed by Restriction Fragment Length Polymorphism (PCR-RFLP) methods. RESULTS: Slow metabolization phenotype was significantly associated as a risk factor for the development of UADT cancer (p=0.038). Furthermore, haplotype of slow metabolization was also associated with UADT cancer (p=0.014). The hOGG1 Ser326Cys polymorphism (CG or GG vs. CC genotypes) was shown as a protective factor against UADT cancer in moderate smokers (p=0.031). The XRCC1 Arg399Gln polymorphism (GA or AA vs. GG genotypes), in turn, was a protective factor against UADT cancer only among never-drinkers (p=0.048). CONCLUSION: Interactions involving NAT2, XRCC1 Arg399Gln and hOGG1 Ser326Cys polymorphisms may modulate the risk of UADT cancer in this population. Copyright
AIM: To evaluate associations between polymorphisms of the N-acetyltransferase 2 (NAT2), human 8-oxoguanine glycosylase 1 (hOGG1) and X-ray repair cross-complementing protein 1 (XRCC1) genes and risk of upper aerodigestive tract (UADT) cancer. PATIENTS AND METHODS: A case-control study involving 117 cases and 224 controls was undertaken. The NAT2 gene polymorphisms were genotyped by automated sequencing and XRCC1 Arg399Gln and hOGG1 Ser326Cys polymorphisms were determined by Polymerase Chain Reaction followed by Restriction Fragment Length Polymorphism (PCR-RFLP) methods. RESULTS: Slow metabolization phenotype was significantly associated as a risk factor for the development of UADT cancer (p=0.038). Furthermore, haplotype of slow metabolization was also associated with UADT cancer (p=0.014). The hOGG1 Ser326Cys polymorphism (CG or GG vs. CC genotypes) was shown as a protective factor against UADT cancer in moderate smokers (p=0.031). The XRCC1 Arg399Gln polymorphism (GA or AA vs. GG genotypes), in turn, was a protective factor against UADT cancer only among never-drinkers (p=0.048). CONCLUSION: Interactions involving NAT2, XRCC1 Arg399Gln and hOGG1 Ser326Cys polymorphisms may modulate the risk of UADT cancer in this population. Copyright