Astrid Collatz Schyum1, Jüri Johannes Rumessen1. 1. Gentofte Hospital HR-Research Unit and Department of Gastroenterology F, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
Abstract
BACKGROUND AND AIM: We present a systematic review on the performance of currently available methods for serological diagnosis of celiac disease (CD) and the role of human leukocyte antigen (HLA) typing. OBJECTIVE: A literature survey was conducted using PubMed, MeSH database, Web of Science as well as manual searches. RESULTS: Tissue transglutaminase antibodies (tTG) (IgA) (tested in nine studies) show sensitivities and specificities in the range of 0.76-0.968 and 0.909-0.98, and deamidated gliadin peptide (DGP) (IgA and IgG) (tested in eight studies) show sensitivities and specificities in the range of 0.69-0.984 and 0.903-1. Endomysial antibodies (EMA) (tested in five studies) show sensitivities and specificities in the range of 0.61-0.937 and 0.98-1, respectively. Combination assays (tested in three studies) using DGP + tTG and DGP (IgA + IgG) show sensitivities and specificities in the range of 0.87-1 and 0.8-1, respectively. HLA DQ2/DQ8 may be necessary for the development of CD-HLA DQ2 in particular. A possible close correlation may also exist between CD and HLA-G. CONCLUSION: DGP and tTG for serological testing for CD show equivalent diagnostic performance. More studies with, in particular, DGP alone and in combination with tTG are necessary before a firm recommendation can be made. HLA typing to exclude CD may still be controversial. It still seems premature to diagnose celiac disease in adults based on serology alone.
BACKGROUND AND AIM: We present a systematic review on the performance of currently available methods for serological diagnosis of celiac disease (CD) and the role of human leukocyte antigen (HLA) typing. OBJECTIVE: A literature survey was conducted using PubMed, MeSH database, Web of Science as well as manual searches. RESULTS:Tissue transglutaminase antibodies (tTG) (IgA) (tested in nine studies) show sensitivities and specificities in the range of 0.76-0.968 and 0.909-0.98, and deamidated gliadin peptide (DGP) (IgA and IgG) (tested in eight studies) show sensitivities and specificities in the range of 0.69-0.984 and 0.903-1. Endomysial antibodies (EMA) (tested in five studies) show sensitivities and specificities in the range of 0.61-0.937 and 0.98-1, respectively. Combination assays (tested in three studies) using DGP + tTG and DGP (IgA + IgG) show sensitivities and specificities in the range of 0.87-1 and 0.8-1, respectively. HLA DQ2/DQ8 may be necessary for the development of CD-HLA DQ2 in particular. A possible close correlation may also exist between CD and HLA-G. CONCLUSION: DGP and tTG for serological testing for CD show equivalent diagnostic performance. More studies with, in particular, DGP alone and in combination with tTG are necessary before a firm recommendation can be made. HLA typing to exclude CD may still be controversial. It still seems premature to diagnose celiac disease in adults based on serology alone.
Authors: Glenn E M Reeves; Marline L Squance; Anne E Duggan; Rajathurai R Murugasu; Robert J Wilson; Richard C Wong; Robert A Gibson; Richard H Steele; Wendy K Pollock Journal: Eur J Gastroenterol Hepatol Date: 2006-05 Impact factor: 2.566
Authors: Emilia Sugai; María L Moreno; Hui J Hwang; Ana Cabanne; Adriana Crivelli; Fabio Nachman; Horacio Vázquez; Sonia Niveloni; Julio Argonz; Roberto Mazure; Graciela La Motta; María E Caniggia; Edgardo Smecuol; Néstor Chopita; Juan C Gómez; Eduardo Mauriño; Julio C Bai Journal: World J Gastroenterol Date: 2010-07-07 Impact factor: 5.742
Authors: Danilo Villalta; Elio Tonutti; Christian Prause; Sibylle Koletzko; H Holm Uhlig; Pieter Vermeersch; Xavier Bossuyt; Martin Stern; Martin W Laass; Julia H Ellis; Paul J Ciclitira; Thomas Richter; Cornelia Daehnrich; Wolfgang Schlumberger; Thomas Mothes Journal: Clin Chem Date: 2009-12-18 Impact factor: 8.327
Authors: Muhammed Hadithi; B Mary E von Blomberg; J Bart A Crusius; Elisabeth Bloemena; Pieter J Kostense; Jos W R Meijer; Chris J J Mulder; Coen D A Stehouwer; Amado S Peña Journal: Ann Intern Med Date: 2007-09-04 Impact factor: 25.391
Authors: Marcis Leja; Zakera Shums; Liene Nikitina-Zake; Mikus Gavars; Ilze Kikuste; Jay Milo; Ilva Daugule; Jelena Pahomova; Valdis Pirags; Vilnis Dzerve; Janis Klovins; Andrejs Erglis; Gary L Norman Journal: United European Gastroenterol J Date: 2015-04 Impact factor: 4.623
Authors: Nicholas A Hoerter; Sarah E Shannahan; Jorge Suarez; Suzanne K Lewis; Peter H R Green; Daniel A Leffler; Benjamin Lebwohl Journal: Dig Dis Sci Date: 2017-02-04 Impact factor: 3.199
Authors: Athena L Sheppard; Martha M C Elwenspoek; Lauren J Scott; Victoria Corfield; Hazel Everitt; Peter M Gillett; Alastair D Hay; Hayley E Jones; Susan Mallett; Jessica Watson; Penny F Whiting Journal: Aliment Pharmacol Ther Date: 2022-01-18 Impact factor: 9.524
Authors: Anna Fowler; Elizabeth J Soilleux; Andrew D Foers; M Saad Shoukat; Oliver E Welsh; Killian Donovan; Russell Petry; Shelley C Evans; Michael Eb FitzPatrick; Nadine Collins; Paul Klenerman Journal: J Pathol Date: 2021-01-06 Impact factor: 9.883