A A S Silva1, S M Morais2, M J C Falcão3, I G P Vieira3, L M Ribeiro3, S M Viana4, M J Teixeira4, F S Barreto5, C A Carvalho6, R P A Cardoso6, H F Andrade-Junior6. 1. Programa de Pós Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Ceará, Brazil. 2. Programa de Pós Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Ceará, Brazil; Curso de Química, Universidade Estadual do Ceará, Ceará, Brazil. Electronic address: selenemaiademorais@gmail.com. 3. Curso de Química, Universidade Estadual do Ceará, Ceará, Brazil. 4. Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Ceará, Brazil. 5. Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Ceará, Brazil. 6. Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
Abstract
UNLABELLED: The aim of the study was to evaluate in vitro the antileishmanial activity of triterpenes and sterols isolated from Musa paradisiaca (banana) fruit peel used traditionally to treat leishmaniasis. The compounds were isolated from the ethanolic extract of the peel of the banana fruit by column chromatography. The chemical structure of compounds was determined by (1)H and (13)C - nuclear magnetic resonance spectroscopy. The cytotoxicity was measured in RAW 264.7 cells and LLC-MK2. Leishmanicidal activity against L. infantum chagasi promastigotes was performed by the MTT colorimetric method and activity against amastigotes was assayed in mammalian cells using in situ ELISA method. Five compounds were identified, consisting of three triterpenes: cycloeucalenone, 31-norcyclolaudenone and 24-methylene-cicloartanol and a mixture of two sterols: beta-sitosterol and stigmasterol. With the exception of cycloeucalenone, all compounds showed statistically similar activity against promastigote to pentamidine. While, acting against amastigotes, excluding 31-norcyclolaudenone, other compounds showed activity similar to amphotericin B. All compounds showed low cytotoxicity in mammalian cells. CONCLUSION: This study partially confirms the use of Musa paradisiaca in folk medicine against leishmaniasis. Further in vivo studies are necessary to evaluate the efficacy.
UNLABELLED: The aim of the study was to evaluate in vitro the antileishmanial activity of triterpenes and sterols isolated from Musa paradisiaca (banana) fruit peel used traditionally to treat leishmaniasis. The compounds were isolated from the ethanolic extract of the peel of the banana fruit by column chromatography. The chemical structure of compounds was determined by (1)H and (13)C - nuclear magnetic resonance spectroscopy. The cytotoxicity was measured in RAW 264.7 cells and LLC-MK2. Leishmanicidal activity against L. infantum chagasi promastigotes was performed by the MTT colorimetric method and activity against amastigotes was assayed in mammalian cells using in situ ELISA method. Five compounds were identified, consisting of three triterpenes: cycloeucalenone, 31-norcyclolaudenone and 24-methylene-cicloartanol and a mixture of two sterols: beta-sitosterol and stigmasterol. With the exception of cycloeucalenone, all compounds showed statistically similar activity against promastigote to pentamidine. While, acting against amastigotes, excluding 31-norcyclolaudenone, other compounds showed activity similar to amphotericin B. All compounds showed low cytotoxicity in mammalian cells. CONCLUSION: This study partially confirms the use of Musa paradisiaca in folk medicine against leishmaniasis. Further in vivo studies are necessary to evaluate the efficacy.
Authors: Daniela Ribeiro Alves; Selene Maia de Morais; Fernanda Tomiotto-Pellissier; Milena Menegazzo Miranda-Sapla; Fábio Roger Vasconcelos; Isaac Neto Goes da Silva; Halisson Araujo de Sousa; João Paulo Assolini; Ivete Conchon-Costa; Wander Rogério Pavanelli; Francisco das Chagas Oliveira Freire Journal: Evid Based Complement Alternat Med Date: 2017-09-07 Impact factor: 2.629
Authors: Patrick O Sakyi; Richard K Amewu; Robert N O A Devine; Emahi Ismaila; Whelton A Miller; Samuel K Kwofie Journal: Nat Prod Bioprospect Date: 2021-07-14