Literature DB >> 24916519

Dynamics of intact immunoglobulin G explored by drift-tube ion-mobility mass spectrometry and molecular modeling.

Kamila J Pacholarz1, Massimiliano Porrini, Rachel A Garlish, Rebecca J Burnley, Richard J Taylor, Alistair J Henry, Perdita E Barran.   

Abstract

Collision cross-sections (CCS) of immunoglobulins G1 and G4 have been determined using linear drift-tube ion-mobility mass spectrometry. Intact antibodies and Fc-hinge fragments present with a larger range of CCS than proteins of comparable size. This is rationalized with MD simulations, which indicate significant in vacuo dynamics between linked folded domains. The IgG4 subclass presents over a wider CCS range than the IgG1 subclass.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords:  conformation analysis; immunoglobulin G; mass spectrometry; molecular dynamics; protein structures

Mesh：

Substances：

Year:  2014        PMID： 24916519     DOI： 10.1002/anie.201402863

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  28 in total

1.  Integrative Mass Spectrometry-Based Approaches for Modeling Macromolecular Assemblies.

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2.  Trapped Ion Mobility Spectrometry of Native Macromolecular Assemblies.

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Journal:  MAbs       Date:  2017-04-13       Impact factor: 5.857

4.  Quantitative collision-induced unfolding differentiates model antibody-drug conjugates.

Authors:  Yuwei Tian; Jennifer L Lippens; Chawita Netirojjanakul; Iain D G Campuzano; Brandon T Ruotolo
Journal:  Protein Sci       Date:  2018-12-22       Impact factor: 6.725

5.  Native Mass Spectrometry, Ion Mobility, Electron-Capture Dissociation, and Modeling Provide Structural Information for Gas-Phase Apolipoprotein E Oligomers.

Authors:  Hanliu Wang; Joseph Eschweiler; Weidong Cui; Hao Zhang; Carl Frieden; Brandon T Ruotolo; Michael L Gross
Journal:  J Am Soc Mass Spectrom       Date:  2019-03-18       Impact factor: 3.109

6.  Coming to Grips with Ambiguity: Ion Mobility-Mass Spectrometry for Protein Quaternary Structure Assignment.

Authors:  Joseph D Eschweiler; Aaron T Frank; Brandon T Ruotolo
Journal:  J Am Soc Mass Spectrom       Date:  2017-07-27       Impact factor: 3.109

7.  Evaluation of Ion Mobility-Mass Spectrometry for Comparative Analysis of Monoclonal Antibodies.

Authors:  Carly N Ferguson; Ashley C Gucinski-Ruth
Journal:  J Am Soc Mass Spectrom       Date:  2016-03-17       Impact factor: 3.109

8.  Native mass spectrometry and ion mobility characterization of trastuzumab emtansine, a lysine-linked antibody drug conjugate.

Authors:  Julien Marcoux; Thierry Champion; Olivier Colas; Elsa Wagner-Rousset; Nathalie Corvaïa; Alain Van Dorsselaer; Alain Beck; Sarah Cianférani
Journal:  Protein Sci       Date:  2015-03-31       Impact factor: 6.725

9.  Signature of Antibody Domain Exchange by Native Mass Spectrometry and Collision-Induced Unfolding.

Authors:  Yasunori Watanabe; Snezana Vasiljevic; Joel D Allen; Gemma E Seabright; Helen M E Duyvesteyn; Katie J Doores; Max Crispin; Weston B Struwe
Journal:  Anal Chem       Date:  2018-05-25       Impact factor: 6.986

Review 10.  Characterization of Pharmaceutical IgG and Biosimilars Using Miniaturized Platforms and LC-MS/MS.

Authors:  Kerry M Wooding; Wenjing Peng; Yehia Mechref
Journal:  Curr Pharm Biotechnol       Date:  2016       Impact factor: 2.837
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