Literature DB >> 24915080

Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones.

Dengfeng Yang1, Takahiro Mori1, Takashi Matsui2, Makoto Hashimoto3, Hiroyuki Morita2, Isao Fujii3, Ikuro Abe1.   

Abstract

CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6-yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P2₁, with unit-cell parameters a=70.0, b=104.8, c=73.5 Å, β=114.4°.

Entities:  

Keywords:  Aspergillus oryzae; CsyB; chalcone synthase; csypyrone B1; polyketide synthase

Mesh：

Substances：

Year:  2014        PMID： 24915080      PMCID： PMC4051524          DOI： 10.1107/S2053230X14008516

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN： 2053-230X            Impact factor:   1.056


  11 in total

Review 1.  The chalcone synthase superfamily of type III polyketide synthases.

Authors:  Michael B Austin; Joseph P Noel
Journal:  Nat Prod Rep       Date:  2003-02       Impact factor: 13.423

Review 2.  Structure and function of the chalcone synthase superfamily of plant type III polyketide synthases.

Authors:  Ikuro Abe; Hiroyuki Morita
Journal:  Nat Prod Rep       Date:  2010-04-01       Impact factor: 13.423

3.  Identification of csypyrone B1 as the novel product of Aspergillus oryzae type III polyketide synthase CsyB.

Authors:  Yasuyo Seshime; Praveen Rao Juvvadi; Katsuhiko Kitamoto; Yutaka Ebizuka; Isao Fujii
Journal:  Bioorg Med Chem       Date:  2010-04-27       Impact factor: 3.641

4.  Structural insights into biosynthesis of resorcinolic lipids by a type III polyketide synthase in Neurospora crassa.

Authors:  Aneesh Goyal; Priti Saxena; Ataur Rahman; Parmit Kumar Singh; Durgadas P Kasbekar; Rajesh S Gokhale; Rajan Sankaranarayanan
Journal:  J Struct Biol       Date:  2008-03-30       Impact factor: 2.867

5.  XDS.

Authors:  Wolfgang Kabsch
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-01-22

6.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

7.  Pentaketide resorcylic acid synthesis by type III polyketide synthase from Neurospora crassa.

Authors:  Nobutaka Funa; Takayoshi Awakawa; Sueharu Horinouchi
Journal:  J Biol Chem       Date:  2007-03-20       Impact factor: 5.157

8.  Identification of csypyrone B2 and B3 as the minor products of Aspergillus oryzae type III polyketide synthase CsyB.

Authors:  Makoto Hashimoto; Yasuyo Seshime; Katsuhiko Kitamoto; Nahoko Uchiyama; Yukihiro Goda; Isao Fujii
Journal:  Bioorg Med Chem Lett       Date:  2012-12-20       Impact factor: 2.823

9.  Aspergillus oryzae type III polyketide synthase CsyA is involved in the biosynthesis of 3,5-dihydroxybenzoic acid.

Authors:  Yasuyo Seshime; Praveen Rao Juvvadi; Katsuhiko Kitamoto; Yutaka Ebizuka; Takamasa Nonaka; Isao Fujii
Journal:  Bioorg Med Chem Lett       Date:  2010-06-25       Impact factor: 2.823

10.  Overview of the CCP4 suite and current developments.

Authors:  Martyn D Winn; Charles C Ballard; Kevin D Cowtan; Eleanor J Dodson; Paul Emsley; Phil R Evans; Ronan M Keegan; Eugene B Krissinel; Andrew G W Leslie; Airlie McCoy; Stuart J McNicholas; Garib N Murshudov; Navraj S Pannu; Elizabeth A Potterton; Harold R Powell; Randy J Read; Alexei Vagin; Keith S Wilson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18
View more
  1 in total

1.  Structural basis for the formation of acylalkylpyrones from two β-ketoacyl units by the fungal type III polyketide synthase CsyB.

Authors:  Takahiro Mori; Dengfeng Yang; Takashi Matsui; Makoto Hashimoto; Hiroyuki Morita; Isao Fujii; Ikuro Abe
Journal:  J Biol Chem       Date:  2015-01-06       Impact factor: 5.157
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.