Ayodele Alaiya1, Lina Assad2, Dania Alkhafaji3, Zakia Shinwari1, Hadeel Almana2, Mohamed Shoukri4, Lutfi Alkorbi3, Hossamaldin Galal Ibrahim3, Mohamed Said Abdelsalam5, Edward Skolnik6, Chaker Adra7, Mamdouh Albaqumi8. 1. Proteomics Unit, Stem Cell and Tissue Re-Engineering Program, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. 2. Department of Pathology, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. 3. Nephrology Section, Department of Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. 4. Department of Biostatistics and Epidemiology, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. 5. Nephrology Section, Department of Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia Internal Medicine Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt. 6. Nephrology Division, Department of Medicine, New York University Medical Center, NY, USA. 7. Proteomics Unit, Stem Cell and Tissue Re-Engineering Program, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia Transplantation Research Center, Renal Division, Children's Hospital Boston and Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. 8. Proteomics Unit, Stem Cell and Tissue Re-Engineering Program, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia Nephrology Section, Department of Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia Nephrology Division, Department of Medicine, New York University Medical Center, NY, USA Nephrology Section, Department of Medicine, MBC-46, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
Abstract
BACKGROUND: There have been several attempts to standardize the definition and increase reproducibility in classifying lupus nephritis (LN). The last was made by the International Society of Nephrology and Renal Pathology Society in 2003 where the introduction of Class IV subcategories (global and segmental) was introduced. METHODS: We investigated whether this subdivision is important using a proteomics approach. All patients with renal biopsies along with their clinical outcome of LN were identified and regrouped according to the above 2003 classifications. Fresh-frozen renal biopsies of Class IV LN (global and segmental), antineutrophil cytoplasmic antibody-associated vasculitis and normal tissue were analyzed using two-dimensional gel electrophoresis (2-DE) and mass spectrometry. Differentially expressed proteins were identified and subjected to principal component analysis (PCA), and post hoc analysis for the four sample groups. RESULTS: PCA of 72 differentially expressed spots separated Class IV global and Class IV segmental from both normal and antineutrophil cytoplasmic antibody-associated vasculitis (ANCA). The 28 identified proteins were used in a post hoc analysis, and showed that IV-global and IV-segmental differ in several protein expression when compared with normal and ANCA. To confirm the proteomic results, a total of 78 patients (50 Class IV-Global and 28 Class IV-Segmental) were re-classified according to 2003 classification. There was no difference in therapy between the groups. The renal survival and patient survivals were similar in both groups. CONCLUSIONS: There is no strong evidence to support a different outcome between the two subcategories of Class-IV LN and, they should thus be treated the same until further studies indicate otherwise.
BACKGROUND: There have been several attempts to standardize the definition and increase reproducibility in classifying lupus nephritis (LN). The last was made by the International Society of Nephrology and Renal Pathology Society in 2003 where the introduction of Class IV subcategories (global and segmental) was introduced. METHODS: We investigated whether this subdivision is important using a proteomics approach. All patients with renal biopsies along with their clinical outcome of LN were identified and regrouped according to the above 2003 classifications. Fresh-frozen renal biopsies of Class IV LN (global and segmental), antineutrophil cytoplasmic antibody-associated vasculitis and normal tissue were analyzed using two-dimensional gel electrophoresis (2-DE) and mass spectrometry. Differentially expressed proteins were identified and subjected to principal component analysis (PCA), and post hoc analysis for the four sample groups. RESULTS: PCA of 72 differentially expressed spots separated Class IV global and Class IV segmental from both normal and antineutrophil cytoplasmic antibody-associated vasculitis (ANCA). The 28 identified proteins were used in a post hoc analysis, and showed that IV-global and IV-segmental differ in several protein expression when compared with normal and ANCA. To confirm the proteomic results, a total of 78 patients (50 Class IV-Global and 28 Class IV-Segmental) were re-classified according to 2003 classification. There was no difference in therapy between the groups. The renal survival and patient survivals were similar in both groups. CONCLUSIONS: There is no strong evidence to support a different outcome between the two subcategories of Class-IV LN and, they should thus be treated the same until further studies indicate otherwise.
Authors: Jessica L Turnier; Hermine I Brunner; Michael Bennett; Ashwaq Aleed; Gaurav Gulati; Wendy D Haffey; Sherry Thornton; Michael Wagner; Prasad Devarajan; David Witte; Kenneth D Greis; Bruce Aronow Journal: Rheumatology (Oxford) Date: 2019-02-01 Impact factor: 7.580
Authors: Rabab Asghar Abdulwahab; Ayodele Alaiya; Zakia Shinwari; Abdul Ameer A Allaith; Hayder A Giha Journal: Int J Mol Med Date: 2019-03-08 Impact factor: 4.101
Authors: Abhimanyu Amarnani; Joseph R Capri; Puneet Souda; David A Elashoff; Ivan A Lopez; Julian P Whitelegge; Ram R Singh Journal: J Proteomics Bioinform Date: 2019-10-03
Authors: Yasser S Basmaeil; Dana Algudiri; Reem Alenzi; Abdullah Al Subayyil; Ayodele Alaiya; Tanvir Khatlani Journal: Stem Cell Res Ther Date: 2020-01-21 Impact factor: 6.832